Stains-All
SIAL/E9379 - ~95%
Synonym: 1-
CAS Number: 7423-31-6
Empirical Formula (Hill Notation): C30H27BrN2S2
Molecular Weight: 559.58
EC Number: 231-047-7
MDL Number: MFCD00078788
Linear Formula: C30H27BrN2S2
Product Type: Chemical
| application(s) | diagnostic assay manufacturing hematology histology |
| assay | ~95% |
| form | powder |
| InChI | 1S/C30H27N2S2.BrH/c1-4-31 |
| InChI key | MPBRYMWMMKKRGC-UHFFFAOYSA |
| Quality Level | 200  |
| SMILES string | [Br-].CCN1C(/Sc2ccc3ccccc |
| solubility | chloroform: 40 mg (PLUS 4.0 ml) |
| storage temp. | 2-8°C |
| Application: | Stains-All has been used for the staining of gel made for size determination of hyaluronan. It has also been used for the staining of DNA oligonucleotides separated by nondenaturing polyacrylamide gel electrophoresis. |
| Biochem/physiol Actions: | Stains-All is a cationic carbocyanine dye and is a multipurpose dye. It stains sialoglycoproteins blue, Ca2+ binding proteins deep blue to violet, proteins red and lipids yellow-orange. It also stains RNA (bluish purple) and DNA (blue). As little as 3 ng (123 BP fragment) of pBR322/Hae III digest DNA and 90 ng tRNA can be detected on a polyacrylamide gel. PAGE gels are stained in the dark and then destaining by removing the gel from the staining solution and exposing it to the light from a light box until sufficient destaining has occurred. A staining solution is typically made by dissolving the dye in formamide and buffer. |
| Caution: | Upon continued exposure to light the nucleic acid-dye complex will fade and disappear. |
| Packaging: | 1, 5 g in glass bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ~95% |
| Storage Temp. | 2-8°C |
| UNSPSC | 12171500 |