Fura 2-AM
SIAL/F0888 - ≥95% (HPLC)
Synonym: Fura-2 pentakis(acetoxymethyl) ester
CAS Number: 108964-32-5
Empirical Formula (Hill Notation): C44H47N3O24
Molecular Weight: 1001.85
MDL Number: MFCD00036976
Linear Formula: C44H47N3O24
Product Type: Chemical
| application(s) | diagnostic assay manufacturing hematology histology |
| assay | ≥95% (HPLC) |
| form | powder |
| InChI | 1S/C44H47N3O24/c1-25-7-8- |
| InChI key | VPSRLGDRGCKUTK-UHFFFAOYSA |
| Quality Level | 100  |
| SMILES string | CC(=O)OCOC(=O)CN(CC(=O)OC |
| solubility | acetone: 10 mg/mL |
| storage temp. | −20°C |
| Application: | Fura 2-AM is a popular probe used to determine dynamic changes in cytosolic free calcium in intact living cells. |
| Features and Benefits: | The major benefits of using the ester form of Fura 2 as Fura 2-AM are: It is cell-permeable. It can be loaded into cells easily. Another advantage of using Fura 2-AM over single wavelength dyes is that the ratio signal is independent of : The dye concentration. Illumination intensity, and Optical path length. |
| General description: | Fura 2-AM (Fura 2) is a calcium-specific ratio-metric fluorescent dye. It is also known as acetoxy-methyl-ester. In response to calcium ions, ratio metric dyes like Fura 2 changes their respective excitation or emission spectra. The intracellular calcium concentration is determined by the fluorescence emission ratio or excitation at distinct wavelengths. Fura 2 has an emission peak at 505nm, and the excitation peak ranges between 340-380nm and is the preferred calcium indicator. |
| Packaging: | 0.5, 1 mg in poly bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (HPLC) |
| Storage Temp. | −20°C |
| UNSPSC | 12171500 |