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Fast Green FCF

SIAL/F7258 - certified by the BSC

Synonym: Food green 3

CAS Number: 2353-45-9
Empirical Formula (Hill Notation): C37H34N2Na2O10S3
Molecular Weight: 808.85
EC Number: 219-091-5
MDL Number: MFCD00013053
Linear Formula: C37H34N2O10S3Na2
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-F7258-25G 25 g
 $236.00
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This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: F7258-25G

 

agency certified by the BSC
application(s) diagnostic assay manufacturing
hematology
histology
composition dye content, ≥90%
form powder
InChI 1S/C37H36N2O10S3.2Na/c1-3-38(24-26-7-5-9-33(21-26)50(41,42)43)30-15-11-28(12-16-30)37(35-20-19-32(40)23-36(35)52(47,48)49)29-13-17-31(18-14-29)39(4-2)25-27-8-6-10-34(22-27)51(44,45)46;;/h5-23H,3-4,24-25H2,1-2H3,(H3,41,42,43,44,45,46,47,48,49);;/q;2*+1/p-2
InChI key RZSYLLSAWYUBPE-UHFFFAOYSA-L
mp 290 °C (dec.) (lit.)
Quality Level 200 
SMILES string [Na+].[Na+].CCN(Cc1cccc(c1)S([O-])(=O)=O)c2ccc(cc2)C(=C3/C=CC(C=C3)=[N+](CC)Cc4cccc(c4)S([O-])(=O)=O)c5ccc(O)cc5S([O-])(=O)=O
solubility H2O: 1 mg/mL
storage temp. room temp
Application: Certified for the safranin-fast green FCF stain for plant cells; as a substitute for aniline blue in Gomori′s one-step trichrome procedure.
Application: Fast Green FCF dye is used for protein staining in IEF, native PAGE and SDS-PAGE. Fast Green staining is linear over a wider range of protein concentrations than Brilliant Blue R. After electrophoresis, fixing the proteins in the gel is recommended for maximum sensitivity. The gel can then be stained for 2 hr with 0.1% Fast Green FCF in either 30% (v/v) ethanol, 10% (v/v) acetic acid or in 7% acetic acid. The gel can be destained with either 30% (v/v) ethanol, 10% (v/v) acetic acid, or 7% acetic acid. The stained gel can be scanned at 625 nm. The detection sensitivity is approx. 30% that with Brilliant Blue R.
Application: Fast Green FCF has been used:
• along with safranin for the staining of nodule sections
• in the collagen deposit staining procedure
• for the staining of protein and starch
• as a dye to detect and inject DNA solution in chicken embryo
Packaging: 25 g in glass bottle
mp 290 °C (dec.) (lit.)
Storage Temp. room temp
Colour Index Number 42053
UNSPSC 12171500

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