Fast Green FCF
SIAL/F7258 - certified by the Biological Stain Commission
Synonym: Food green 3
CAS Number: 2353-45-9
Empirical Formula (Hill Notation): C37H34N2Na2O10S3
Molecular Weight: 808.85
EC Number: 219-091-5
MDL Number: MFCD00013053
Linear Formula: C37H34N2O10S3Na2
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: F7258-25G
| application(s) | diagnostic assay manufacturing hematology histology |
| composition | dye content, ≥90% |
| form | powder |
| grade | certified by the Biological Stain Commission |
| InChI | 1S/C37H36N2O10S3.2Na/c1-3 |
| InChI key | RZSYLLSAWYUBPE-UHFFFAOYSA |
| mp | 290 °C (dec.) (lit.) |
| Quality Level | 200  |
| SMILES string | [Na+].[Na+].CCN(Cc1cccc(c |
| solubility | H2O: 1 mg/mL |
| storage temp. | room temp |
| Application: | Fast Green FCF dye is used for protein staining in IEF, native PAGE and SDS-PAGE. Fast Green staining is linear over a wider range of protein concentrations than Brilliant Blue R. After electrophoresis, fixing the proteins in the gel is recommended for maximum sensitivity. The gel can then be stained for 2 hr with 0.1% Fast Green FCF in either 30% (v/v) ethanol, 10% (v/v) acetic acid or in 7% acetic acid. The gel can be destained with either 30% (v/v) ethanol, 10% (v/v) acetic acid, or 7% acetic acid. The stained gel can be scanned at 625 nm. The detection sensitivity is approx. 30% that with Brilliant Blue R. |
| Application: | Fast Green FCF has been used: • along with safranin for the staining of nodule sections • in the collagen deposit staining procedure • for the staining of protein and starch • as a dye to detect and inject DNA solution in chicken embryo |
| Packaging: | 25 g in glass bottle |
| Suitability: | Certified for the safranin-fast green FCF stain for plant cells; as a substitute for aniline blue in Gomori′s one-step trichrome procedure. |
| Symbol |  GHS08 |
| Signal word | Warning |
| Hazard statements | H341 |
| Precautionary statements | P201 - P202 - P280 - P308 + P313 - P405 - P501 |
| Hazard Codes | Xn |
| Risk Statements | 68 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| mp | 290 °C (dec.) (lit.) |
| Storage Temp. | room temp |
| Colour Index Number | 42053 |
| UNSPSC | 12171500 |