Folin & Ciocalteu’s phenol reagent
SIAL/F9252 - suitable for determination of total protein by Lowry method, 1.9-2.1 N
MDL Number: MFCD00132625
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: F9252-100ML.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: F9252-1L.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: F9252-500ML.
| application(s) | diagnostic assay manufacturing hematology histology |
| color | clear yellow |
| faint yellow to very dark yellow, and Faint Green-Yellow to Very Dark Green-Yellow | |
| concentration | 1.9-2.1 N |
| density | 1.240 g/cm3 at 20 °C |
| form | liquid |
| pH | <0.5 (20 °C) |
| Quality Level | 200  |
| storage temp. | room temp |
| suitability | suitable for determination of total protein by Lowry method |
| Application: | Folin & Ciocalteu’s phenol reagent has been used for the estimation of total phenolic content in samples. |
| General description: | Folin & Ciocalteu′s phenol reagent is most commonly used in the Lowry method for determining protein concentration. It has also been used for the quantification of total phenolics. In this method, protein is pretreated with copper(II) in a modified biuret reagent (alkaline copper solution stabilized with sodium potassium tartrate). Addition of the phenol reagent generates chromogens that give increasing absorbance between 550-750nm. Normally, absorbance at the peak (750nm) or shoulder (660nm) are used to quantitate protein concentrations between 1-100 mg/ml while absorbance at 550nm is used to quantitate higher protein concentrations. In the absence of copper, color intensity is determined primarily by the tyrosine and tryptophan content of the protein, and to a lesser extent by cysteine and histidine. Copper(II) has no effect on color formation by tyrosine, tryptophan, or histidine, but reduces color formation due to cysteine. Many modifications of the original assay procedure have been published, including methods for enhancing the color development, for determining the content of insoluble proteins, and for automating the procedure. Compounds including many buffers, chelating agents, detergents, and cyclic organic compounds can interfere with the Lowry protein assay. Folin & Ciocalteu′s phenol reagent can also be used as a spray reagent in chromatographic procedures. |
| Linkage: | This product is also available as part of a kit. |
| Other Notes: | Folin & Ciocalteu′s phenol reagent does not contain phenol. Rather, the reagent will react with phenols and non-phenolic reducing substances to form chromogens that can be detected spectrophotometrically. |
| Packaging: | 1 L in glass bottle |
| Packaging: | 100, 500 mL in glass bottle |
| Symbol |  GHS05 |
| Signal word | Danger |
| Hazard statements | H290 - H314 |
| Precautionary statements | P234 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363 |
| Hazard Codes | C,Xi |
| Risk Statements | 35-36/38 |
| Safety Statements | 26-36/37/39-45 |
| RIDADR | UN 3264 8 / PGIII |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Density | 1.240 g/cm3 at 20 °C |
| Storage Temp. | room temp |
| UNSPSC | 12171500 |