HEPES
SIAL/H23830 - reagent grade, ≥99% (titration), crystalline
Synonym: 4-
CAS Number: 7365-45-9
Empirical Formula (Hill Notation): C8H18N2O4S
Molecular Weight: 238.30
MDL Number: MFCD00006158
Linear Formula: C8H18N2O4S
Product Type: Chemical
| assay | ≥99% (titration) |
| biological source | synthetic |
| form | crystalline |
| grade | reagent grade |
| InChI | 1S/C8H18N2O4S/c11-7-5-9-1 |
| InChI key | JKMHFZQWWAIEOD-UHFFFAOYSA |
| pKa (25 °C) | 7.5 |
| Quality Level | 200  |
| SMILES string | OCCN1CCN(CC1)CCS(O)(=O)=O |
| useful pH range | 6.8-8.2 |
| Application: | HEPES has been used: • As a component in Danieau medium, which is used to prevent melanin pigment formation post gastrulation of zebra-fish embryo† • As a component of Hanks′ solution used in the degradation testing of Iron† • As a component of HEPES medium in the transfection of cell culture with plasmids† • Along with RPMI-1640, L-glutamine, FBS, Sodium pyruvate and β-mercaptoethanol for the culturing of rat insulinoma cells† • As a supplement in TCM199 medium for washing fresh oocytes required for nuclear transfer studies† |
| General description: | HEPES has been described as one of the best all-purpose buffers available for biological research.[7] At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.[1] |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥99% (titration) |