Hematoxylin
SIAL/H3136 - certified by the BSC
Synonym: Natural Black 1
CAS Number: 517-28-2
Empirical Formula (Hill Notation): C16H14O6 · xH2O
Molecular Weight: 302.28 (anhydrous basis)
EC Number: 208-237-3
MDL Number: MFCD00078111
Linear Formula: C16H14O6 · xH2O
Product Type: Chemical
| agency | certified by the BSC |
| application(s) | diagnostic assay manufacturing hematology histology |
| form | powder |
| InChI | 1S/C16H14O6/c17-10-2-1-8- |
| InChI key | WZUVPPKBWHMQCE-XJKSGUPXSA |
| mp | 200 °C (dec.) (lit.) |
| Quality Level | 200 ![]() |
| SMILES string | Oc1cc2C[C@@]3(O)COc4c(O)c |
| solubility | ethanol: 1 mg/mL |
| storage temp. | room temp |
| Application: | Certified for use in Weigert′s iron hematoxylin, Mayer′s hemalum, Harris hemalum and in Heidenhain′s technique |
| Application: | Hematoxylin has been used: • to visualize anatomical features and to detect nucleus locus coeruleus adjacent to the periaqueductal gray region in brainstem samples • as a nuclear counterstain to visualize the nucleus, adipocytes were counterstained with a hematoxylin reagent • to stain lung tissue for histological analysis |
| General description: | Hematoxylin is a basophilic dye extracted from the tree Heamatoxylum campechianum. It specifically binds to acidic compounds and thus is useful for staining cell nuclei which contain acidic substances: DNA and RNA. Hematoxylin, when oxidized forms hematein, which has thick pale blue purple colour. Hematoxylin is often used along with eosin, in histological study under light microscope. |
| Packaging: | 25, 100 g in glass bottle |
| Symbol | GHS07 |
| Signal word | Warning |
| Hazard statements | H319 |
| Precautionary statements | P264 - P280 - P305 + P351 + P338 - P337 + P313 |
| Hazard Codes | Xn |
| Risk Statements | 22-36/37/38 |
| Safety Statements | 26-36 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| mp | 200 °C (dec.) (lit.) |
| Storage Temp. | room temp |
| Colour Index Number | 75290 |
| UNSPSC | 12171500 |


