A549 HLA Panel Cell Lines
SIAL/HLA003
Product Type: Chemical
| biological source | human lung (Carcinoma) |
| growth mode | Adherent |
| morphology | Epithelial |
| shipped in | dry ice |
| storage temp. | −196°C |
| Cell Line Description: | Derived from a 58 year old Caucasian male. The cells can synthesise lecithin utilising the cytidine diphosphocholine pathway. Occasional cells may also contain inclusion bodies although they are not known to carry any human pathogen. |
| Components: | HLA003A-1VL: A549 B2M KO Cells HLA003B-1VL: A549 HLA-A*02:01 Cells HLA003C-1VL: A549 HLA-A*01:01 Cells HLA003D-1VL: A549 HLA-A*03:01 Cells HLA003E-1VL: A549 HLA-A*11:01 Cells HLA003F-1VL: A549 HLA-A*24:02 Cells HLA003G-1VL: A549 HLA-B*15:10 Cells HLA003H-1VL: A549 HLA-B*07:02 Cells HLA003I-1VL: A549 HLA-B*08:01 Cells HLA003J-1VL: A549 HLA-B*35:01 Cells HLA003K-1VL: A549 HLA-B*40:01 Cells |
| Features and Benefits: | Unlike wild type A549 cells, which express multiple HLA subtypes, the A549 HLA panel cell lines express individual MHC Class I HLA subtypes. These monoallelic HLA cell lines can be used to study peptide binding and presentation in the context of individual HLA subtypes. The panel can also be used to develop cell-based assays to evaluate candidate cancer immunotherapies and identify tumor-specific neoantigens. |
| General description: | The STR profile of these cell lines match that of its parental cell line - ECACC catalog number 86012804. This product is a panel of eleven (11) genetically modified cell lines targeting MHC Class I HLA molecules in an A549 lung carcinoma parental cell line background. The full HLA panel consists of a Beta-2 microglobulin (β2M) knockout cell line and an additional ten (10) monoallelic HLA expression cell lines. CompoZr® Zinc Finger Nuclease (ZFN) technology was used to generate the β2M knockout cell line, which does not express endogenous cell surface MHC Class I HLA molecules (HLA-A or HLA-B) as measured by fluorescence-activated cell sorting (FACS). MISSION® lentivirus was used to generate the ten (10) monoallelic HLA cell lines, which express individual HLA-A or HLA-B subtypes on the cell surface via a β2M:HLA fusion protein as described in Nature Biotechnology 35, 765-772, (2017) and as demonstrated via FACS analysis. |
| Legal Information: | MISSION Use Licenses Agreement  ZFN Use Licenses Agreement |
| Legal Information: | CompoZr is a registered trademark of Sigma-Aldrich Co. LLC |
| Legal Information: | MISSION is a registered trademark of Sigma-Aldrich Co. LLC |
| Quality: | Tested for Mycoplasma, bacterial and fungal content, post-freeze viability, and short terminal repeat (STR) analysis for cell line identification. |
| WGK Germany | WGK 2 |
| Storage Temp. | −196°C |