Isopropyl β-D-1-thiogalactopyranoside
SIAL/I5502 - ≥99% (TLC)
Synonym: IPTG; Isopropyl β-D-thiogalactoside
CAS Number: 367-93-1
Empirical Formula (Hill Notation): C9H18O5S
Molecular Weight: 238.30
EC Number: 206-703-0
MDL Number: MFCD00063273
Linear Formula: C9H18O5S
Product Type: Chemical
IPTG induction mechanism illustration. Lac promoters are under the control of Lac repressor and is activated only in the presence of lactose or its synthetic analog IPTG. Like lactose, IPTG binds the repressor and releases his binding to the promoter, but unlike lactose, it′s not metabolized. The release of the repressor allows the transcription of the target gene.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: I5502-1G
This image depicts SKU: I5502-1G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: I5502-10G
This image depicts SKU: I5502-10G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: I5502-5G
This image depicts SKU: I5502-5G
| assay | ≥99% (TLC) |
| color | white to off-white |
| description | non-ionic |
| form | powder |
| impurities | 10.0-22.0% Dioxane |
| InChI | 1S/C9H18O5S/c1-4(2)15-9-8 |
| InChI key | BPHPUYQFMNQIOC-NXRLNHOXSA |
| mp | 120 -122 °C ((248 - 252 °F )) |
| Quality Level | 200  |
| SMILES string | CC(C)S[C@@H]1OC(CO)[C@H]( |
| solubility | water: 50 mg/mL, clear, colorless |
| storage temp. | 2-8°C |
| technique(s) | thin layer chromatography (TLC): suitable |
| Application: | IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ. |
| Other Notes: | Non-metabolizable galactose analog. |
| Other Notes: | To gain a comprehensive understanding of our extensive range of Monosaccharides for your research, we encourage you to visit our Carbohydrates Category page. |
| Packaging: | 1, 5, 10 g in poly bottle |
| Reconstitution: | A stock solution (0.1 M) is prepared by dissolving IPTG in water with subsequent sterile filtration of the solution. The final concentration of IPTG in indicator plates should be 0.2 mM. |
| Symbol |   GHS07,GHS08 |
| Signal word | Warning |
| Hazard statements | H319 - H351 |
| Precautionary statements | P281 - P305 + P351 + P338 |
| Hazard Codes | Xn |
| Risk Statements | 40 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥99% (TLC) |
| mp | 120 -122 °C ((248 - 252 °F )) |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352201 |