IPTG
SIAL/I6758 - ≥99% (TLC), ≤0.1% Dioxane
Synonym: Isopropyl β-D-1-thiogalactopyranoside; IPTG; Isopropyl β-D-thiogalactoside
CAS Number: 367-93-1
Empirical Formula (Hill Notation): C9H18O5S
Molecular Weight: 238.30
EC Number: 206-703-0
MDL Number: MFCD00063273
Linear Formula: C9H18O5S
Product Type: Chemical
IPTG induction mechanism illustration. Lac promoters are under the control of Lac repressor and is activated only in the presence of lactose or its synthetic analog IPTG. Like lactose, IPTG binds the repressor and releases his binding to the promoter, but unlike lactose, it′s not metabolized. The release of the repressor allows the transcription of the target gene.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: I6758-10G
| application(s) | cell analysis life science and biopharma |
| assay | ≥99% (TLC) |
| biological source | synthetic |
| color | white to off-white |
| description | non-ionic |
| form | powder |
| impurities | ≤0.1% Dioxane |
| InChI | 1S/C9H18O5S/c1-4(2)15-9-8 |
| InChI key | BPHPUYQFMNQIOC-NXRLNHOXSA |
| mp | 105 °C ((221 °F)) |
| Quality Level | 300  |
| SMILES string | CC(C)S[C@@H]1OC(CO)[C@H]( |
| solubility | water: 0.05 g/L, clear |
| storage temp. | 2-8°C |
| technique(s) | thin layer chromatography (TLC): suitable |
| useful pH range | 5-7 (50 g/L) |
| Application: | IPTG has been used: • in the induction of protein expression in Lactobacillus reuteri GroEL and mutants • in the induction of protein expression in Escherichia coli |
| Application: | IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ. |
| Biochem/physiol Actions: | IPTG is a non-metabolizable analog of allolactose. It acts as an inducer that regulates the transcriptional activity of the lac promoter, a promoter that is used for the expression of the protein in E.coli. |
| General description: | Isopropyl β-D-Thiogalactopyranoside (IPTG) is a molecular biology reagent used to induce gene expression in bacterial systems. It mimics lactose, binding to the lac repressor protein, leading to gene activation. IPTG is crucial for studying gene regulation, protein expression, and recombinant protein production, providing valuable insights into cellular and molecular biology research. |
| Other Notes: | For additional information on our range of Biochemicals , please complete this form . |
| Other Notes: | Non-metabolizable galactose analog. |
| Other Notes: | To gain a comprehensive understanding of our extensive range of Monosaccharides for your research, we encourage you to visit our Carbohydrates Category page. |
| Packaging: | 1, 5, 10 g in glass bottle |
| Reconstitution: | A stock solution (0.1 M) is prepared by dissolving IPTG in water with subsequent sterile filtration of the solution. The final concentration of IPTG in indicator plates should be 0.2 mM. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥99% (TLC) |
| mp | 105 °C ((221 °F)) |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352201 |