Invertase from baker's yeast (S. cerevisiae)
SIAL/I9274 - 200-300 units/mg solid
Synonym: β-D-Fructofuranosidase; β-D-Fructofuranoside fructohydrolase; Saccharase
CAS Number: 9001-57-4
EC Number: 232-615-7
MDL Number: MFCD00131410
Product Type: Chemical
| application(s) | food and beverages general analytical |
| foreign activity | α-glucosidase (maltase), phosphoglucomutase, phosphoglucose isomerase ≤0.01% |
| 6-phosphogluconic dehydrogenase, α-galactosidase, β-galactosidase, and NADH oxidase ≤0.001% | |
| Quality Level | 100  |
| specific activity | 200-300 units/mg solid |
| storage temp. | 2-8°C |
| technique(s) | photometry: suitable |
| Application: | Invertase from baker′s yeast (S. cerevisiae) has been used: • in liquid media (YES) and is used to determine the invertase activity • supplemented to the reaction mixture in the extraction and analysis of sugars and starch • for sucrose hydrolysis |
| Application: | Used in the production of confectionary foods and artificial honey. |
| Biochem/physiol Actions: | Invertase hydrolyzes sucrose into glucose and fructose yielding a colorless product, unlike acid hydrolysis which produces colored products. |
| General description: | Invertase is encoded by the SUC2 (Saccharomyces cerevisiae S288C) gene. In yeast this enzyme exist in: glycosylated (secreted) and non-glycosylated (intracellular) form. The external invertase is composed of carbohydrate (50%) and 60,000 D subunits. |
| Packaging: | 100 mg in poly bottle |
| Unit Definition: | One unit will hydrolyze 1.0 μmole sucrose to glucose and fructose per minute at pH 4.6 at 25 °C. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| activity | specific activity: 200-300 units/mg solid |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.2.1.26 ( BRENDA  | IUBMB ) |
| UNSPSC | 12164500 |