Kanamycin sulfate from Streptomyces kanamyceticus
SIAL/K4000 - Animal Component-free
Synonym: Kanamycin A; Kanamycin sulfate salt
CAS Number: 25389-94-0
Empirical Formula (Hill Notation): C18H36N4O11 · H2O4S
Molecular Weight: 582.58
EC Number: 246-933-9
MDL Number: MFCD00070253
Linear Formula: C18H36N4O11 · H2O4S
Product Type: Chemical
antibiotic activity spectrum | Gram-negative bacteria |
Gram-positive bacteria | |
mycobacteria | |
mycoplasma | |
biological source | Streptomyces kanamyceticus |
color | white to off-white |
form | powder |
impurities | ≤5% Kanamycin B |
InChI | 1S/C18H36N4O11.H2O4S/c19- |
InChI key | OOYGSFOGFJDDHP-KMCOLRRFSA |
mode of action | protein synthesis | interferes |
potency | ≥750 μg per mg (dry basis) |
Quality Level | 300 ![]() |
SMILES string | OS(O)(=O)=O.NC[C@H]1O[C@H |
solubility | H2O: 50 mg/mL, clear, colorless to faintly yellow |
Application: | Kanamycin sulfate is a broad spectrum aminoglycoside-antibiotic derived from Streptomyces kanamyceticus. It is used as an additive in culture media for the isolation of group D streptococci on Kanamycin Esculin Azide Agar and for selection of transformed plant cells containing the neomycin phosphotransferase on a kanamycin-medium. Kanamycin sulfate can also be used as a selection agent for cells transformed with kanamycin B resistance gene. It is recommended for use in cell culture applications at 100 μg/mL. |
Biochem/physiol Actions: | Mode of Action: The product acts by binding to the 70S ribosomal subunit, inhibiting translocation and eliciting miscoding. Mode of Resistance:Aminoglycoside Antimicrobial spectrum: Kanamycin sulfate is effective against gram-negative and gram-postiive bacteria, and mycoplasma. |
Caution: | Solutions are stable at 37°C for approximately 5 days. Aqueous stock solutions can be stored at 2-8°C for long term storage. |
General description: | Chemical structure: aminoglycoside |
Packaging: | 1, 5, 25, 50 g in poly bottle |
Preparation Note: | Kanamycin sulfate is soluble in water at 50 mg/mL, yielding a clear solution. It is practically insoluble in alcohol, acetone, chloroform, ether and ethyl acetate. A 1% solution in water has a pH of 6.5 to 8.5. Sterile solutions can be prepared by a sterile filtration, through a .2μm filter. |
Symbol | ![]() |
Signal word | Danger |
Hazard statements | H360D |
Precautionary statements | P201 - P202 - P280 - P308 + P313 - P405 - P501 |
Hazard Codes | T |
Risk Statements | 61 |
Safety Statements | 53-45 |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 2 |
UNSPSC | 51101500 |