Lysine medium
SIAL/L5910 - suitable for microbiology, NutriSelect® Plus
Product Type: Chemical
| application(s) | food and beverages |
| microbiology | |
| final pH | 5.0±0.2 (25 °C) |
| form | powder |
| manufacturer/tradename | NutriSelect® Plus |
| product line | BioChemika |
| Quality Level | 200  |
| shelf life | limited shelf life, expiry date on the label |
| sterility | non-sterile |
| suitability | nonselective for Candida spp. |
| nonselective for Pichia spp. | |
| nonselective for Saccharomyces spp. | |
| nonselective for Zygosaccharomyces spp. | |
| technique(s) | microbe id | utilization test: suitable |
| microbiological culture: suitable |
| Application: | Used for isolation and enumeration of wild yeasts in pitching yeasts. |
| Components: | Ingredients (g/L) Dextrose, 44.50 Monopotassium phosphate, 1.78 Magnesium sulphate, 0.89 Calcium chloride, 0.178 Sodium chloride, 0.089 Adenine, 0.00178 Boric acid, 0.0000089 Zinc sulphate, 0.0000356 Ammonium molybdate, 0.0000178 Manganese sulphate, 0.0000356 Ferrous sulphate, 0.0002225 Inositol, 0.02 Calcium pantothenate, 0.002 Aneurine, 0.0004 Pyridoxine, 0.0004 p-Aminobenzoic acid, 0.0002 Nicotinic acid, 0.0004 Riboflavin, 0.0002 Biotin, 0.000002 Folic acid, 0.000001 Agar, 17.80 |
| Footnote: | We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance. |
| Legal Information: | GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Preparation Note: | Suspend 6.6 g of Lysine Medium in 100 ml of distilled water containing 0.84 ml of potassium lactate solution ~60% (Cat. No. 60389). Boil to dissolve the medium completely. Cool to 50 °C. Adjust the pH to 5.0 with a 10% solution of Lactic Acid (Cat. No. 69778) and pour into sterile petri plates.NoteThe degree of contamination is expressed as the number of wild yeast cells per million cells of the original inoculum. The number of cells in the inoculum is important as a small number (100-1000) grow to a limited extent while 10′000 brewing yeast cells provide a direct measure of contaminant wild yeasts. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 41171606 |