Middlebrook 7H9 Broth Base
SIAL/M0178 - suitable for microbiology, NutriSelect® Plus,
Recommended for use in isolation and cultivation of Mycobacterium species
Synonym: Mycobacterial culture media
Product Type: Chemical
| application(s) | clinical testing food and beverages life science and biopharma veterinary |
| microbiology | |
| final pH | 6.6±0.2 (25 °C) |
| form | powder |
| manufacturer/tradename | NutriSelect® Plus |
| product line | BioChemika |
| Quality Level | 200  |
| shelf life | limited shelf life, expiry date on the label |
| sterility | non-sterile |
| storage temp. | 2-25°C |
| suitability | mycobacteria |
| technique(s) | microbe id | susceptibility testing: suitable |
| microbiological culture: suitable |
| Application: | Middlebrook 7H9 Broth Base has been used for the cultivation Mycobacterium bovis bacillus Calmette-Guérin Pasteur strain. It is also recommended for cultivation and sensitivity testing of Mycobacterium tuberculosis. |
| Components: | Ingredients (g/L) Ammonium sulfate, 0.50 Disodium phosphate, 2.50 Monopotassium phosphate, 1.00 Sodium citrate, 0.10 Magnesium sulfate, 0.05 Calcium chloride, 0.0005 Zinc sulfate, 0.001 Copper sulfate, 0.001 Ferric ammonium citrate, 0.04 Pyridoxine, 0.001 Biotin, 0.0005 |
| Footnote: | We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance. |
| General description: | Middlebrook 7H9 Broth Base contains inorganic compounds that supply the elements potassium, sulfur, magnesium, and phosphorus which are required for appropriate growth of Mycobacteria. This medium with Middlebrook ADC Growth Supplement is recommended for ideal growth of Mycobacterium species. |
| Legal Information: | GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | TWEEN is a registered trademark of Croda International PLC |
| Preparation Note: | Suspend 2.35 g of Middlebrook 7H9 Broth Base in 450 ml of distilled water. Add either 2 ml of glycerol (Cat. No 49767) or 0.5 g of TWEEN® 80 (Cat. No. 93780). Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. Pressure (121°C) for 10 minutes. Cool to 45°C or below and aseptically add 1 vial of Middlebrook ADC Growth Supplement (Cat. No. M0553). Mix well before dispensing. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-25°C |
| UNSPSC | 41171607 |