Middlebrook 7H9 Broth Base

SIAL/M0178 - suitable for microbiology, NutriSelect^® Plus,
Recommended for use in isolation and cultivation of Mycobacterium species

Synonym: Mycobacterial culture media

Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-M0178-500G	500 g	$264.00 1/EA	Add To Favorites

This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: M0178-500G

Properties
Descriptions
Safety Info.
Basic Data

application(s)	clinical testing food and beverages life science and biopharma veterinary
	microbiology
final pH	6.6±0.2 (25 °C)
form	powder
manufacturer/tradename	NutriSelect^® Plus
product line	BioChemika
Quality Level	200
shelf life	limited shelf life, expiry date on the label
sterility	non-sterile
storage temp.	2-25°C
suitability	mycobacteria
technique(s)	microbe id \| susceptibility testing: suitable
	microbiological culture: suitable

Application:	Middlebrook 7H9 Broth Base has been used for the cultivation Mycobacterium bovis bacillus Calmette-Guérin Pasteur strain. It is also recommended for cultivation and sensitivity testing of Mycobacterium tuberculosis.
Components:	Ingredients (g/L) Ammonium sulfate, 0.50 Disodium phosphate, 2.50 Monopotassium phosphate, 1.00 Sodium citrate, 0.10 Magnesium sulfate, 0.05 Calcium chloride, 0.0005 Zinc sulfate, 0.001 Copper sulfate, 0.001 Ferric ammonium citrate, 0.04 L-Glutamic acid, 0.50 Pyridoxine, 0.001 Biotin, 0.0005
Footnote:	We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
General description:	Middlebrook 7H9 Broth Base contains inorganic compounds that supply the elements potassium, sulfur, magnesium, and phosphorus which are required for appropriate growth of Mycobacteria. This medium with Middlebrook ADC Growth Supplement is recommended for ideal growth of Mycobacterium species. Middlebrook ADC Growth Supplement (Product No. M0553) contains bovine albumin, dextrose, and catalase. Dextrose supplies energy, while albumin protects Mycobacteria from toxic agents and catalase splits toxic radicals. Mycobacteria grow most rapidly in broth media and hence primary isolation of bacterial species can be carried out in Middlebrook 7H9 Broth Base.
Legal Information:	GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
Legal Information:	NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Legal Information:	TWEEN is a registered trademark of Croda International PLC
Preparation Note:	Suspend 2.35 g of Middlebrook 7H9 Broth Base in 450 ml of distilled water. Add either 2 ml of glycerol (Cat. No 49767) or 0.5 g of TWEEN^® 80 (Cat. No. 93780). Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. Pressure (121°C) for 10 minutes. Cool to 45°C or below and aseptically add 1 vial of Middlebrook ADC Growth Supplement (Cat. No. M0553). Mix well before dispensing.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Storage Temp.	2-25°C
UNSPSC	41171607

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