Middlebrook 7H10 Agar Base
SIAL/M0303 - suitable for microbiology, NutriSelect® Plus, for use in isolation and cultivation of Mycobacterium species
Synonym: Mycobacterial culture media
Product Type: Chemical
| application(s) | clinical testing food and beverages life science and biopharma veterinary |
| microbiology | |
| final pH | 6.6±0.2 (25 °C) |
| form | powder |
| manufacturer/tradename | NutriSelect® Plus |
| packaging | pkg of 500 g |
| product line | BioChemika |
| Quality Level | 200  |
| shelf life | limited shelf life, expiry date on the label |
| sterility | non-sterile |
| suitability | mycobacteria |
| technique(s) | microbe id | susceptibility testing: suitable |
| microbiological culture: suitable |
| Application: | Middlebrook 7H10 Agar Base is a selective media for isolation, cultivation and sensitivity testing of Mycobacterium tuberculosis. Mycobacteria grow most rapidly in broth media and hence primary isolation of bacterial species can be carried out. It is inexpensive, easy to prepare and can aid in early detection of acid-fast, Mycobacterium species by culture. |
| Components: | Ingredients (g/L) Ammonium sulfate, 0.50 Monopotassium phosphate, 1.50 Disodium phosphate, 1.50 Sodium citrate, 0.40 Ferric ammonium citrate, 0.04 Magnesium sulfate, 0.025 Calcium chloride, 0.0005 Zinc sulfate, 0.001 Copper sulfate, 0.001 Pyridoxine hydrochloride, 0.001 Biotin, 0.0005 Malachite green, 0.00025 Agar, 15.00 |
| Footnote: | We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance. |
| General description: | Middlebrook 7H10 Agar Base is recommended for isolation, cultivation and sensitivity testing of Mycobacterium tuberculosis. Middlebrook media consists of many inorganic salts, which help, in growth of Mycobacteria. Citric acid formed from sodium citrate helps in retaining inorganic cations in solution. Glycerol supplies carbon and energy. Middlebrook OADC Growth Supplement (M0678) contains oleic acid, bovine albumin, sodium chloride, dextrose and catalase. Oleic acid and other long chain fatty acids are essential for metabolism of Mycobacteria. Some free fatty acids are toxic to Mycobacteria, but albumin binds to those fatty acids and prevents toxic action on Mycobacteria. Dextrose serves as an energy source. Catalase neutralizes toxic peroxides. Malachite green partially inhibits other bacteria. |
| Legal Information: | GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Preparation Note: | Suspend 19.47 g of Middlebrook 7H10 Agar Base in 900 ml of distilled water. Add 5 ml of glycerol (Cat. No. 49767). Boil to dissolve the medium completely. Distribute in 180 ml amounts in erlenmeyer flasks and sterilize by autoclaving at 15 lbs. pressure (121°C) for 10 minutes. Cool to 45°C and aseptically add 20 ml of Middlebrook OADC Growth Supplement (Cat. No. M0678). Mix well and pour into screw capped tubes.Note: Keep prepared medium in the dark before and after inoculation. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 41171609 |