Middlebrook 7H11 Agar Base
SIAL/M0428 - suitable for microbiology, NutriSelect® Plus
Synonym: Media for culturing Mycobacteria
Product Type: Chemical
| application(s) | clinical testing food and beverages life science and biopharma veterinary |
| microbiology | |
| final pH | 6.6±0.2 (25 °C) |
| form | powder |
| manufacturer/tradename | NutriSelect® Plus |
| product line | BioChemika |
| Quality Level | 200  |
| shelf life | limited shelf life, expiry date on the label |
| sterility | non-sterile |
| storage condition | protect from light at room temperature |
| suitability | mycobacteria |
| technique(s) | microbe id | susceptibility testing: suitable |
| microbiological culture: suitable |
| Application: | There are two types of solid culture media for the primary isolation of Mycobacteria. Egg-based (Lowenstein’s formulation) and agar-based (Middlebrook formulation) The latter has the following advantages over the egg-based media • The Middlebrook 7H11 agar base does not liquify easily in the presence of contaminating proteolytic organisms • There is less inactivation of added drugs when egg ingredients are absent |
| Components: | Ingredients (g/L) Casein enzymatic hydrolysate, 1.00 Ammonium sulfate, 0.50 Monopotassium phosphate, 1.50 Disodium phosphate, 1.50 Sodium citrate, 0.40 Magnesium sulfate, 0.05 Ferric ammonium citrate, 0.04 Pyridoxine, 0.001 Biotin, 0.0005 Malachite green, 0.001 Agar, 15.00 |
| Footnote: | We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance. |
| General description: | Middlebrook 7H11 Agar Base media is used for the isolation, cultivation, and sensitivity testing of Mycobacterium species. This agar base contains many inorganic salts which help the growth of Mycobacteria. Citric acid formed from sodium citrate helps in retaining inorganic cations in solution. Glycerol supplies carbon and energy. The Middlebrook OADC Supplement (M0678) contains oleic acid, bovine albumin, sodium chloride, dextrose and catalase. Oleic acid and other long chain fatty acids are essential for the metabolism of Mycobacteria. Dextrose is an energy source. Catalase neutralizes toxic peroxides. Albumin protects tubercle bacilli from toxic agents. Malachite green partially inhibits other bacteria. |
| Legal Information: | GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Preparation Note: | Suspend 10.25 g of Middlebrook 7H11 Agar Base in 450 ml of distilled water. Add 2.5 ml of glycerol (Cat. No. 49767). Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Cool to 50°C and aseptically add 1 vial Middlebrook OADC Growth Supplement (M0678). Mix thoroughly before dispensing. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 41171609 |