Methyl Green
SIAL/M8884 - zinc chloride salt, ~85%
Synonym: Ethyl Green
CAS Number: 7114-03-6
Empirical Formula (Hill Notation): C27H35BrClN3 · ZnCl2
Molecular Weight: 653.24
EC Number: 230-415-4
MDL Number: MFCD00151094
Linear Formula: C27H35BrClN3 · ZnCl2
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: M8884-25G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: M8884-5G
| εmax | >11100.00 at 417-425 nm in water at 0.005 g/L |
| >16000 at 311-319 nm in water at 0.005 g/L | |
| >60000 at 626-634 nm in water at 0.005 g/L | |
| application(s) | diagnostic assay manufacturing hematology histology |
| assay | ~85% |
| color | red to very dark red |
| composition | Dye Content, ≥83% |
| form | powder |
| InChI | 1S/C27H35N3.BrH.3ClH.Zn/c |
| InChI key | RRKSQWOQZFIQKW-UHFFFAOYSA |
| mp | >300 °C (lit.) |
| pH | 0.2-1.8, yellow to blue |
| Quality Level | 200  |
| SMILES string | [Cl-].[Br-].Cl[Zn]Cl.CC[N |
| solubility | water: 1 mg/mL |
| storage temp. | room temp |
| technique(s) | titration: suitable |
| Analysis Note: | Dye content based on molecular weight of monobromo-monochloro ZnCl2 salt (653.2). |
| Analysis Note: | Material may be a mixture of monobromo-monochloro and dichloro ZnCl2 salts. Halogen contents listed on label. |
| Application: | Methyl green is suitable as a nuclear counterstain in enzyme histochemistry, immunostaining, and in situ hybridization as the bluish-green color will be in contrast with red, purple, brown, and black products of histochemical reactions. It is also suitable as a tracking dye in Acid-polyacrylamide gel electrophoresis to separate gliadins. It has been used in the histochemical staining for the apoptosis examination of kidney tissue and as a nuclear stain for labeling embryos. |
| General description: | Methyl green is a synthetic, triphenylmethane-type cationic dye related to ethyl green mainly used as DNA stain in histochemistry. It possesses aniline rings with different degrees of methylation. The dye binds specifically to the A-T rich regions of the major groove of DNA with the help of two positive charges. Methyl green in combination with pyronin is extensively used to stain RNA and plasma cells in tissue sections and cytologic preparations. |
| Packaging: | 25 g in glass bottle |
| Packaging: | 5 g in poly bottle |
| Symbol |    GHS05,GHS07,GHS09 |
| Signal word | Danger |
| Hazard statements | H314 - H335 - H411 |
| Precautionary statements | P260 - P271 - P273 - P280 - P303 + P361 + P353 - P305 + P351 + P338 |
| Hazard Codes | Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 26-36 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Purity | ~85% |
| mp | >300 °C (lit.) |
| Storage Temp. | room temp |
| Colour Index Number | 42590 |
| UNSPSC | 12171500 |