Methylene blue
SIAL/M9140 - certified by the Biological Stain Commission
Synonym: Basic Blue 9; 3,7-
CAS Number: 122965-43-9
Empirical Formula (Hill Notation): C16H18ClN3S · xH2O
Molecular Weight: 319.85 (anhydrous basis)
EC Number: 200-515-2
MDL Number: MFCD00150006
Linear Formula: C16H18ClN3S · xH2O
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: M9140-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: M9140-25G
| application(s) | diagnostic assay manufacturing hematology histology |
| color | dark green |
| composition | Dye content, ≥82% |
| form | powder |
| grade | certified by the Biological Stain Commission |
| InChI | 1S/C16H18N3S.ClH.H2O/c1-1 |
| InChI key | WQVSELLRAGBDLX-UHFFFAOYSA |
| Quality Level | 200  |
| SMILES string | CN(C)C1=CC=C2C(SC(C3=N2)= |
| solubility | H2O: 4mg/4ml |
| storage temp. | room temp |
| Application: | Methylene blue has been used: • to stain vaginal cells • for the preparation of protein aqueous ink • to evaluate cell survival by staining |
| Biochem/physiol Actions: | Methylene blue is used as a bacteriologic stain or dye and indicator. It mediates the inactivation of enveloped viruses in plasma, when exposed to visible light. It is an inhibitor of nitric oxide synthase and guanylate cyclase. It also plays a key role in medicine as an antimalarial agent, reducing agent in methemoglobinemia and inducer of apoptosis in cancer cells. |
| General description: | Methylene blue is a compound that occurs as crystals or crystalline powder, having a bronze-like luster. It is soluble in water, chloroform and partially soluble in alcohol. It is a photoactive dye, which is a member of phenothiazine family. |
| Packaging: | 25, 100 g in glass bottle |
| Suitability: | Certified for use in Mallory′s phloxine-methylene blue stain; Levine & Black stain for bacteria in milk; Wright stain for blood smears. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H302 |
| Precautionary statements | P264 - P270 - P301 + P312 - P501 |
| Hazard Codes | Xn |
| Risk Statements | 22-36/37/38 |
| Safety Statements | 26-36 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | room temp |
| Colour Index Number | 52015 |
| UNSPSC | 12171500 |