Methotrexate

SIAL/M9929 - meets USP testing specifications

Synonym: Methotrexate hydrate; 4-Amino-10-methylfolic acid hydrate; L-4-Amino-N¹⁰-methylpteroylglutamic acid hydrate; L-Amethopterin hydrate; Antifolan hydrate; MTX hydrate; Methylaminopterin hydrate

CAS Number: 133073-73-1
Empirical Formula (Hill Notation): C₂₀H₂₂N₈O₅ · xH₂O
Molecular Weight: 454.44 (anhydrous basis)
MDL Number: MFCD00150847
Linear Formula: C₂₀H₂₂N₈O₅ · xH₂O
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-M9929-25MG	25 mg	$107.00 1/EA	Add To Favorites
45-M9929-100MG	100 mg	$285.00 1/EA	Add To Favorites
45-M9929-500MG	500 mg	$1120.00 1/EA	Add To Favorites

This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.

This image depicts SKU: M9929-100MG

Properties
Descriptions
Safety Info.
Basic Data

agency	meets USP testing specifications
	USP/NF
antibiotic activity spectrum	parasites
application(s)	advanced drug delivery metabolomics microbiology
assay	98.0-102.0%
biological source	synthetic
color	yellow to orange-yellow
form	powder
impurities	≤0.1% Residue on ignition (Ash)
	≤12.0% water (Karl Fischer)
InChI	1S/C20H22N8O5.H2O/c1-28(9-11-8-23-17-15(24-11)16(21)26-20(22)27-17)12-4-2-10(3-5-12)18(31)25-13(19(32)33)6-7-14(29)30;/h2-5,8,13H,6-7,9H2,1H3,(H,25,31)(H,29,30)(H,32,33)(H4,21,22,23,26,27);1H2/t13-;/m0./s1
InChI key	FPJYMUQSRFJSEW-ZOWNYOTGSA-N
mode of action	DNA synthesis
mp	185-204 °C (365 - 399 °F)
Quality Level	400
SMILES string	[H]O[H].CN(Cc1cnc2nc(N)nc(N)c2n1)c3ccc(cc3)C(=O)N[C@@H](CCC(O)=O)C(O)=O
solubility	water: insoluble
storage condition	(Tightly closed. Dry. Keep in a well-ventilated place. )
storage temp.	−20°C
technique(s)	activity assay: suitable
	cell based assay: suitable

Application:	Potent inhibitor of dihydrofolate reductase and agent for antitumor studies. Use to inhibit dihydrofolate reductase in DHFR-based protein expression systems. Also effective in treatment of pyrimethamine-resistant Plasmodium vivax malaria parasites.
Application:	Potent inhibitor of dihydrofolate reductase and agent for antitumor studies. Use to inhibit dihydrofolate reductase in DHFR-based protein expression systems. Also shows immunosuppressive effects in, e.g., rheumatoid arthritis.
Biochem/physiol Actions:	Methotrexate is an allosteric inhibititor of dihydrofolate reductase (DHFR), the enzyme that catalyzes the conversion of dihydrofolate to tetrahydrofolate. Since tetrahydrolfolate is required for purine and pyrimidine synthesis, methotrexate treatment results in the inhibition of DNA and RNA synthesis.
Other Notes:	For additional information on our range of Biochemicals , please complete this form .
Packaging:	25, 100, 500 mg in poly bottle

Symbol	GHS06,GHS08
Signal word	Danger
Hazard statements	H301 - H315 - H319 - H360FD
Precautionary statements	P201 - P202 - P264 - P301 + P310 - P302 + P352 - P305 + P351 + P338
Hazard Codes	T
Risk Statements	61-25-36/38
Safety Statements	53-26-36/37-45
RIDADR	UN 1544PSN1 6.1 / PGIII
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	98.0-102.0%
mp	185-204 °C (365 - 399 °F)
Storage Temp.	−20°C
UNSPSC	12352005

To Order From a Quote