Legionella in Water PT - WP
SIAL/MIC004 - Proficiency Testing Material
Product Type: Chemical
| application(s) | environmental food and beverages |
| matrix | Vitroid™ |
| packaging | set of 2 ea |
| Quality Level | 100  |
| shipped in | wet ice |
| storage temp. | −20°C |
| suitability | Legionella spp. |
| Application: | Legionella in Water - Water Pollution (WP) proficiency testing material is designed for the quantitative determination of Legionella bacteria in wastewater samples. Have any questions? Explore our FAQs . For more information on this product, please contact our PT Service Team . |
| Features and Benefits: | We have a vast portfolio of environmental PT schemes with "fit-for-purpose" samples to help you meet the needs of the laboratory. Our proficiency testing (PT) program is a benchmark to: • provide staff with an insight into their laboratory′s performance, to verify the accuracy and reliability of their testing process • provide a comparison of performance with that of other laboratories, nationally and internationally • test and educate staff, provide a better understanding of the impact of incorrect results and identify areas where there may be problems • provide an independent evaluation of laboratory performance • demonstrate to clients, colleagues and accreditation bodies that there is a commitment to quality |
| General description: | Legionella in Water - Water Pollution (WP) is produced in accordance with ISO/IEC 17043:2010. The PT portfolio allows our test components to offer significant performance data to monitor and prove competency to customers, accreditation bodies or both. Supelco® is a global proficiency testing (PT) provider that manufactures best-in-class environmental and pharmaceutical PT products. Most water PT samples are available as concentrates or ready-to-use volumes giving the end-user flexibility in selecting samples that will fit their exact needs. Our WP program is designed to meet the quality assurance requirements, thereby ensuring the analytical competence of your laboratory. These samples are typically provided in duplicate for use in case of lab mishap, for QC purposes or to analyze multiple methods. Type of PT Scheme: Water analysis - Water Pollution (WP) Matrix: Water Pollution/Wastewater Analyte/s: Values of analytes vary lot to lot. All information regarding the use of Legionella in Water - Water Pollution (WP) proficiency testing standard can be found in the reporting packet which will be available to download at PT Portal . |
| Legal Information: | Supelco is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | Vitroid is a trademark of Sigma-Aldrich Co. LLC |
| Other Notes: | Are you new to Supelco® Proficiency Testing? Be sure to register your account details in the PT Portal before placing your order. This will provide access to data entry, reporting packets and final reports. Registrations will be approved shortly after your order is placed. For further questions, write to us at ptservice@milliporesigma. When placing your order online, please indicate your study preference under the ′your reference′ column. Available studies can be viewed at the bottom of the page. Click here to learn more about Quick-Turn Studies (on-demand proficiency tests that are offered depending on lot availability). The Quick-Turn results are released within 48-72 hours whereas scheduled study reports are released within 21 days.Proficiency testing samples are presented as single blind. No values for the samples are released until the testing period concludes. Proficiency Testing Schedule (2022-2023) Wastewater WP22-2 March 23 - May 6, 2022 WP22-4 September 21 - November 4, 2022 WP23-2 March 22 – May 5, 2023 WP23-4 September 20 – November 3, 2023 Quick-Turn Study (on demand proficiency test that is offereddepending on lot availability) |
| Packaging: | Set contains 2 sterile screw cap vials each containing a Vitroid (a small disk containing the possible organisms) and 2 - 99mL bottles of phosphate buffer. (Please note, buffer solution may not be provided with your sample. If this causes any concern, please contact PT Service ptservice@milliporesigma. |
| Preparation Note: | Description• This PT sample is designed for the quantitative analysis of Legionella in water. The PT sample is provided in duplicate as a stable dehydrated disc requiring hydration. The additional sample is provided for mishaps in the lab. This sample requires 100 ml of buffer/ saline solution for hydration fluid (see note below). The dehydrated disc sample is stable for a time exceeding the study schedule. General Information• Due to the sensitivity of microorganisms and short holding times after hydration, please read entire instructions prior to preparation. This sample is a single disc and the possible concentration range is quite broad, it may be necessary to analyze the sample in a dilution series using general phosphate buffer solution. The hydration temperature of 35-37°C is rigorous. Low temperature will not assure complete hydration. Higher temperature will kill the microorganisms. Sample Preparation• Allow the capped sample vial to equilibrate to room temperature. Open the sample vial and aseptically transfer the disc to the hydrating fluid. • The sample is the colored disc within the cryotube. It will either be on top of cotton with opaque desiccant beads below the cotton or free moving in a cryotube that contains a desiccant tablet within the cap. After 15 min inspect to assure dissolution of the disc. It should dissolve completely. This represents the sample for analysis. Dilutions are recommended to achieve a proper count, but base all counts on the whole volume sample. NOTE: The Vitroid™ is the colored disk within the cryotube. It will either be on top of cotton with opaque desiccant beads below the cotton or free moving in a cryotube that contains a desiccant tablet within the cap. The opaque desiccant beads and desiccant tablet are to keep the container dry and are not biologicals. • Shake well and place the sample in your incubator for 15 minutes. The hydration temperature of 35 - 37 °C is rigorous. • Low temperature will not assure complete hydration. • Higher temperatures will kill the microorganisms. After 15 minutes, remove the hydrated sample from the incubator and shake well. Inspect to assure dissolution of the Vitroid™. The disk will dissolve completely. • This represents the sample for analysis. Dilutions are recommended to achieve a proper count but base all counts on the whole volume sample. Note: We are no longer supplying Phosphate buffer for Micro samples. Please proceed to use your laboratory′s standard buffer or saline solution. If your laboratory does not have buffer in-house, we recommend using P3813, Phosphate Powder which can be purchased at SigmaAldrich.com. |
| Storage and Stability: | Please refer to the sample label for storage conditions. |
| RIDADR | UN3373 - class 6.2 Biological substance, Category B |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |
| Components | Legionella 0.00 - 50000 CFU/mL |