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WS-Enterococci PT-Sample (1-10)

SIAL/MIC016 - Proficiency Testing Material

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-MIC016-10EA 10 ea
$357.00
1/EA
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application(s) environmental
food and beverages
format matrix material
matrix Vitroid
packaging set of 10 ea
Quality Level 100 
shipped in wet ice
storage temp. −20°C
suitability Enterococcus spp.
Analysis Note: This product was designed, produced and verified for accuracy and stability in accordance with ISO/IEC 17025:2005, ISO GUIDES 34 and 35, and ISO/IEC 17043.
Application: Water Supply (WS) - Enterococci PT - Sample (1 - 10) proficiency testing material is designed for the qualitative determination of Enterococci in water supply/drinking water samples.

Have any questions? Explore our FAQs .
For more information on this product, please contact our PT Service Team .
Features and Benefits: We have a vast portfolio of environmental PT schemes with "fit-for-purpose" samples to help you meet the needs of the laboratory.

Our proficiency testing (PT) program is a benchmark to:

• provide staff with an insight into their laboratory′s performance, to verify the accuracy and reliability of their testing process
• provide a comparison of performance with that of other laboratories, nationally and internationally
• test and educate staff, provide a better understanding of the impact of incorrect results and identify areas where there may be problems
• provide an independent evaluation of laboratory performance
• demonstrate to clients, colleagues and accreditation bodies that there is a commitment to quality

General description: Water Supply (WS) - Enterococci PT - Sample (1 - 10) is produced in accordance with ISO/IEC 17043:2010. The PT portfolio allows our test components to offer significant performance data to monitor and prove competency to customers, accreditation bodies or both.

Supelco® is a global proficiency testing (PT) provider that manufactures best-in-class environmental and pharmaceutical PT products. Most water PT samples are available as concentrates or ready-to-use volumes giving the end-user flexibility in selecting samples that will fit their exact needs. Our WS program is designed to meet the quality assurance requirements in compliance with the Safe Drinking Water Act (SDWA), thereby ensuring the analytical competence of your laboratory or drinking water facility.

Type of PT Scheme: Water analysis - Water Supply (WS)

Matrix: Water Supply/Drinking water

Analyte/s: Values of analytes vary lot to lot.

All information regarding the use of WS - Enterococci PT - Sample (1-10) proficiency testing standard can be found in the reporting packet which will be available to download at PT Portal .
Legal Information: Supelco is a registered trademark of Merck KGaA, Darmstadt, Germany
Legal Information: Vitroid is a trademark of Sigma-Aldrich Co. LLC
Other Notes: Are you new to Supelco® Proficiency Testing? Be sure to register your account details  in the PT Portal before placing your order.
This will provide access to data entry, reporting packets and final reports. Registrations will be approved shortly after your order is placed. For further questions, write to us at ptservice@milliporesigma.com.

When placing your order online, please indicate your study preference under the ′your reference′ column. Available studies can be viewed at the bottom of the page.
Click here  to learn more about Quick-Turn Studies (on-demand proficiency tests that are offered depending on lot availability). The Quick-Turn results are released within 48-72 hours whereas scheduled study reports are released within 21 days.

Proficiency Testing Schedule (2022-2023)

Drinking Water
WS22-2 March 23 - May 6, 2022

WS23-2 March 22 – May 5, 2023

Quick-Turn Study (on demand proficiency test that is offereddepending on lot availability)


This sample set contains 10 vials each containing a dehydrated tablets containing possible organisms. Each sample vial is uniquely marked with a sample number. The vials containing the dehydrated sample must be stored as directed. The dehydrated Vitroid sample is stable for a time exceeding the study schedule.
Packaging: Set contains 10 sterile screw cap vials each containing a Vitroid (a small disk containing the possible organisms) and 10 - 99mL bottles of phosphate buffer.
(Please note, buffer solution may not be provided with your sample. If this causes any concern, please contact PT Service ptservice@milliporesigma.com.)
Preparation Note: Description

• This sample set contains 10 vials each containing a dehydrated tablet containing possible organisms. Ten sterile 100 mL phosphate buffered hydrolyzing solutions are also provided. (Please note, buffer solution may not be provided with your sample. If this causes any concern, please contact PT Service ptservice@milliporesigma.com.) Each sample vial is uniquely marked with a sample number and color coded cap (Samples 1, 6 - Clear, Sample 2, 7 - Red, Sample 3, 8 - Blue, Sample 4, 9 - Yellow, Samples 5, 10 - Green.) The buffer bottles are NOT labeled. Remember to label the buffer bottles prior to mixing.
• The sample is the colored disk. If the disk is lodged in the cap, drop entire cap into the hydration fluid.
• Ensure the disk has dissolved completely (no color remains) and aseptically remove the cap prior to analysis. The hydrolyzing fluid may be stored at room temperatures.
• The bacteria are provided in dehydrated form for enhanced stability. They must be re-hydrated prior to performing the tests. The vials holding the bacteria are sterile in the sealed box. Once the box is opened it is recommended hydration and analysis is started immediately.
• Each disk contains a high concentration of bacteria. Before you begin, make certain that appropriate methods and media are being used. Label each dilution bottle with the sample number, the study number prior to hydrolyzing the samples. All of these numbers can be found on the vial labels. Make sure to use a permanent marker that is water insoluble.
• Do not transfer any disks into hydrolyzing liquid prior to warming it. If you are using an enzyme substrate test follow manufacturer′s instructions. If the result is questionable at the end of the incubation period, you may incubate for an additional 4 hours or as provided in the method.
• It is important that you report values for Enterococci. You must report all samples to receive an evaluation. We are not allowed to make any assumptions so please fill out your forms carefully.

Preparation Instructions

• Remove the sample vials and dilution bottles from refrigeration and allow them to equilibrate to room temperature.
• Open the bottle and aseptically transfer a dehydrated tablet to the bottle.
• Label the bottle, cap and shake gently. The tablet will dissolve completely in about 15 minutes.
• Repeat the above procedure with each sample. Due to the sensitivity of the organisms, analysis should be completed with in 1 hour of hydration.
• Each sample will contain a concentration of bacteria from 20 to 500 CFU/100 mL.
• You may dilute the sample with phosphate buffer to meet your method needs.

Reporting Instructions

Analyze the sample by your normal procedures and report results for each sample as present (“1”) or absent (“0”). If you enumerate, report in the space provided for MPN or CFU/100mL.


Storage and Stability: • Please refer to sample label for expiration date, specific storage temperature, and specific storage conditions.
• If storage conditions are not provided on the label, it is intended for room temperature storage.
RIDADR UN3373 - class 6.2 Biological substance, Category B
WGK Germany WGK 1
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Storage Temp. −20°C
UNSPSC 12352200

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800-334-7725
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