E.coli in Seawater - Quantitative WP
SIAL/MIC022
Product Type: Chemical
| application(s) | environmental food and beverages |
| format | matrix material |
| matrix | Vitroid™ |
| packaging | set of 2 ea |
| Quality Level | 100  |
| shipped in | wet ice |
| storage temp. | −20°C |
| suitability | Escherichia coli |
| coliforms |
| Analysis Note: | This product was designed, produced and verified for accuracy and stability in accordance with ISO/IEC 17025:2005, ISO GUIDES 34 and 35, and ISO/IEC 17043. |
| Application: | E. coli in Seawater - Quantitative WP is a non-Potable water (NPW) PT standard, suitable for testing E. coli in seawater. Have any questions? Explore our FAQs .For more information on this product, please contact our PT Service Team . |
| Features and Benefits: | We have a vast portfolio of environmental PT schemes with "fit-for-purpose" samples to help you meet the needs of the laboratory. Our proficiency testing (PT) program is a benchmark to: • provide staff with an insight into their laboratory′s performance, to verify the accuracy and reliability of their testing process • provide a comparison of performance with that of other laboratories, nationally and internationally • test and educate staff, provide a better understanding of the impact of incorrect results and identify areas where there may be problems • provide an independent evaluation of laboratory performance • demonstrate to clients, colleagues and accreditation bodies that there is a commitment to quality |
| General description: | E. coli in Seawater - Quantitative WP proficiency testing material is produced in accordance with ISO/IEC 17043:2010. The PT portfolio allows our test components to offer significant performance data to monitor and prove competency to customers, accreditation bodies or both. Supelco® is a global proficiency testing (PT) provider that manufactures best-in-class environmental and pharmaceutical PT products. Most water PT samples are available as concentrates or ready-to-use volumes giving the end-user flexibility in selecting samples that will fit their exact needs. Our WP program is designed to meet the quality assurance requirements, thereby ensuring the analytical competence of your laboratory. These samples are typically provided in duplicate for use in case of lab mishap, for QC purposes or to analyze multiple methods. Type of PT Scheme: Water analysis - Water Pollution (WP) Matrix: Water Pollution/Wastewater Analyte/s: Values of analytes vary lot to lot. All information regarding the use of E. coli in Seawater - Quantitative WP can be found in the reporting packet which will be available to download at PT Portal . |
| Legal Information: | Supelco is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | Vitroid is a trademark of Sigma-Aldrich Co. LLC |
| Other Notes: | Are you new to Supelco® Proficiency Testing? Be sure to register your account details in the PT Portal before placing your order. This will provide access to data entry, reporting packets and final reports. Registrations will be approved shortly after your order is placed. For further questions, write to us at ptservice@milliporesigma. When placing your order online, please indicate your study preference under the ′your reference′ column. Available studies can be viewed at the bottom of the page. Click here to learn more about Quick-Turn Studies (on-demand proficiency tests that are offered depending on lot availability). The Quick-Turn results are released within 48-72 hours whereas scheduled study reports are released within 21 days.This PT sample is designed for the quantitative analysis of total and/or fecal coliform and E. coli in seawater. The PT sample is provided in duplicate as a stable dehydrated Vitroid™ requiring hydration. The additional sample is provided for mishaps in the lab. Store the PT sample as directed. The dehydrated Vitroid™ sample is stable for a time exceeding the study schedule. Proficiency testing samples are presented as single blind. No values for the samples are released until the testing period concludes. Proficiency Testing Schedule (2022-2023) Wastewater WP22-2 March23 - May 6, 2022 WP22-4 September21 - November 4, 2022 WP23-2 March 22 – May 5, 2023 WP23-4 September20 – November 3, 2023 Quick-Turn Study (on demand proficiency test that is offereddepending on lot availability) |
| Packaging: | Set contains 2 sterile screw cap vials each containing a Vitroid™ (a small disk containing the possible organisms) and 2 - 99mL bottles of phosphate buffer. (Please note, buffer solution may not be provided with your sample. If this causes any concern, please contact PT Service ptservice@milliporesigma. |
| Preparation Note: | Description • This PT sample is designed for the quantitative analysis of total and/or fecal coliform and E. coli in seawater. • The PT sample is provided in duplicate as a stable dehydrated Vitroid™ requiring hydration. An additional sample is provided for mishaps in the lab. TNI prohibits the practice of multiple tests on a PT sample unless it is normal laboratory practice and multiple analysis is conducted on a normal day to day samples. • A 100 mL of sterile hydration fluid is supplied with the PT sample. (Please note, buffer solution may not be provided with your sample. If this causes any concern, please contact PT Service ptservice@milliporesigma. • The dehydrated Vitroid™ sample is stable for a time exceeding the study schedule. Sample Preparation • Allow the capped sample vial to equilibrate to room temperature. • While the sample is equilibrating to room temperature, warm the hydrating fluid in your incubator to 35 - 37 °C. This will take about 60 min. This step is not necessary but will aid the dissolution of the Vitroid™. If you do not have the capability to warm the hydration fluid, allow it to reach room temperature prior to starting the next step. The hydration fluid is sterile and may be stored at room temperature up to the expiration date listed on the bottom, refrigeration is not necessary for the hydration fluid. • Open the sample vial and aseptically transfer the Vitroid™ to the hydrating fluid. NOTE: The Vitroid™ is the colored disk on top of the cotton. The opaque beads below the cotton keep the container dry and are not biologicals. • Allow the Vitroid™ to dissolve completely. This may take up to 15 min. Mix by inverting gently several times, shaking may cause the cap to leak. • This represents the sample for analysis. Incubate at the temperature required by your method. Temperature requirements are rigorous, higher temperatures will kill the organisms and lower temperatures may suppress growth. The minimum incubation time is 24 h. Further incubation may be necessary depending on the method and media. Further dilutions are recommended to achieve a proper count, but base all counts on the whole volume sample. If you have questions please contact ptservice@milliporesigma. |
| Storage and Stability: | Please refer to sample label for expiration date, specific storage temperature, and specific storage conditions. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |
| Components | Total coliforms 20.0 - 2400 CFU/100mL; Total Coliform, MPN 20.0 - 2400 MPN/100 mL; Escherichia coli 20.0 - 2400 CFU/100mL; Escherichia coli, MPN 20.0 - 2400 MPN/100 mL; Fecal coliforms 20.0 - 2400 CFU/100mL; Fecal coliform, MPN 20.0 - 2400 MPN/100 mL |