Caco-2 Engineered Control Cells
SIAL/MTOX1000CC24 - one assay ready, 24-well plate
Product Type: Chemical
| biological source | human male colorectal tissue (Source Disease: adenocarcinoma) |
| form | liquid |
| Application: | The Engineered Control cells can be used for drug permeability assays. They may also be used as the control in conjunction with C2BBe1 Transporter Knockout cell lines for drug/transporter interaction studies. Engineered control cells have been used in the in vitro Caco-2 permeability assay. The following posters and articles demonstrate how Caco-2 cells can be used for cell based assays: Transporter Function in Caco-2 Cells with Targeted P-Glycoprotein, MRP2 and BCRP Gene Knockout Using Zinc Finger Nucleases  Comparison of Function and Relative Transporter Protein Concentrations in Caco-2 Cells with Single and Double Knockouts of the ABCB1, ABCG2, and ABCC2 Genes Caco-2 Transporter Knockout Cell Based Assays |
| Features and Benefits: | The Caco-2 subclone, C2BBe1 Engineered Control cells, are ideal for permeability and transporter analyses as they express multiple transporters, are human derived and grow in a homogenous monolayer that forms tight junctions which is necessary for efflux ratio analysis. Other benefits include: • The 24-well Transwell format enables the these cells to be included in standard drug transporter protocols • Human assay with no interference from animal inhibitors • The 21-day cell culture period is reduced to 6-days for customers, saving valuable resources |
| General description: | The C2BBe1 cells, a sublcone of Caco-2 cells, correspond to ATCC CRL-2101. The C2BBe1 cells are adenocarcinoma, epithelial cells from a human caucasian male (aged 72 years). The three week production lead time begins on the Monday following a purchase, in the third week the plates are shipped on Tuesday for receipt on Wednesday or Thursday. As a biologic product that is shipped at room temperature the cells must be processed immediately upon receipt. A minimum of 4 assay ready plates is required to purchase. Please allow for 1 week after ordering, for the plates to be seeded. Plates will be shipped to the customer on day 16 of the 21-day culturing process. |
| Legal Information: | This product is covered under a consumable purchase agreement for one-time use only. For more information:ADME/Tox Cell Lines License |
| Quality: | Tested for Mycoplasma, bacterial and fungal content, post-freeze viability, short terminal repeat (STR) analysis for cell line identification. |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 41106514 |