Kidney PTEC OCT2 KO Cells
SIAL/MTOX1080
Product Type: Chemical
Brightfield image of RPTEC cells in 2D culture following 5 days of growth
RPTEC in 2D monolayer: confocal image of ZO-1, a marker of tight junctions, staining
RPTEC in 2D monolayer: confocal image of Actin staining (Phalloidin)
RPTEC in 2D monolayer: confocal image of Acetylated tubulin, present on the cilia and an indicator of polarization
RPTEC in 2D monolayer: confocal image of Nuclear stain
Brightfield image of RPTEC in 2D culture spontaneously forming tubules
Confocal image showing RPTEC (blue, nuclear stain) forming a tubule while retaining dye (green) in the lumen - a mark of a functional barrier
Confocal image showing RPTEC on the AIM Biochip (DAX01). RPTEC form a tight, polarized barrier as indicated by ZO-1 (green), Actin (purple), Acetylated Tubulin (red), and nuclear (blue) staining
Confocal image showing RPTEC on the Mimetas OrganoPlate® (Z742750). RPTEC form a polarized barrier as indicated by ZO-1 (green), Actin (purple), Acetylated Tubulin (red), and nuclear (blue) staining
Confocal image showing RPTEC on the Mimetas OrganoPlate® (Z742750). RPTEC form a polarized tubule in the channel
| biological source | human female kidney (proximal convoluted tubule epithelial cells) |
| description | 1 vial |
| form | liquid |
| OMIM accession no. | 602608   |
| Quality Level | 200 |
| storage temp. | −196°C |
| Application: | Human RPTEC SA7K control cell line and transporter KO models have been validated in several standard and 3D assays for measuring nephrotoxicity and transporter assays. RPTEC SA7K control cells can be used to measure toxicity of compounds using dose-response curves and viability such as MTT based in vitro Toxicology in both 2D and 3D culture. RPTEC SA7K control cells can also be used to measure compound uptake/efflux by transporters using inhibitors. Precision editing in RPTEC SA7K transporter KO cells provides a more predictive platform for compound uptake/efflux by transporters without the need for non-specific inhibitors. RPTEC SA7K cells have been validated for use on the Mimetas 3-lane OrganoPlate®in both toxicity and barrier integrity assays( Z742750 ). RPTEC SA7K cells can also be used on the AIM Biotech 3D cell culture chip in toxicity and permeability assays ( DAX01 ). |
| Features and Benefits: | Sigma′s human RPTEC control SA7K clone cells and knockout (KO) cells exhibit normal renal proximal tubule characteristics, functional renal uptake and efflux transporters and are sensitive to human nephrotoxicants making them ideal for studying drug-transporter interactions and early detection of renal toxicants. The SA7K clone should be used as a control for Sigma′s human RPTEC KO cells. Sigma′s human RPTEC OCT2 Knockout (KO) allows investigations of drug transporter interactions, uptake, and clearance. |
| General description: | Sigma modified human primary renal proximal tubule epithelial cells (RPTEC) with ZFNs to effectively create novel human RPTEC cell lines that can be utilized for investigating kidney toxicology (nephrotoxicity), transporters and various other renal studies. Sigma′s RPTEC′s exhibit normal epithelial morphology and expression of Renal Proximal Tubule (PT) specific markers such as, CD13 and a-GST. Sigma′s RPTEC′s maintain typical PT functions including albumin reabsorption, ?-glutamyl transpeptidase (GGT) activity, and respond to parathyroid hormone. The cells respond to several known human nephrotoxicants similar to human primary cells and demonstrate robust expression and activity of key kidney uptake and efflux transporters incuding OCT2, OATP4C1, OAT1, OCTN1, MRP2, MRP4, P-gp, MATE1, MATE2-K, PEPT1, and PEPT2. |
| Legal Information: | OrganoPlate is a registered trademark of MIMETAS B.V. |
| Legal Information: | These products are covered by the ADME/Tox Cell Lines License as described in Exhibit 1, in the technical bulletin. |
| Quality: | Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation. |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −196°C |
| UNSPSC | 41106514 |