Nitrotetrazolium Blue chloride
SIAL/N6639 - powder, electrophoresis grade
Synonym: 2,2′-bis(4-Nitrophenyl)-5,5′-diphenyl-3,3′-(3,3′-dimethoxy-4,4′-diphenylene)ditetrazolium chloride; 3,3′-(3,3′-Dimethoxy-4,4′-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride]; p-Nitro-Blue tetrazolium chloride; p-Nitrotetrazolium blue; NBT; Nitro BT
CAS Number: 298-83-9
Empirical Formula (Hill Notation): C40H30Cl2N10O6
Molecular Weight: 817.64
EC Number: 206-067-4
MDL Number: MFCD00012159
Linear Formula: C40H30N10O6 · 2Cl
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: N6639-250MG.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: N6639-50MG.
| assay | >90% |
| form | powder |
| grade | electrophoresis grade |
| InChI | 1S/C40H30N10O6.2ClH/c1-55 |
| InChI key | FSVCQIDHPKZJSO-UHFFFAOYSA |
| Quality Level | 100  |
| SMILES string | [Cl-].[Cl-].COc1cc(ccc1-[ |
| solubility | 2-methoxyethanol: 20 mg/mL |
| ethanol: 5 mg/mL | |
| H2O: 10 mg/mL (A stock solution at 10mg/ml in water is stable for 1-2 weeks in the dark at 0-4°C.) | |
| storage temp. | 2-8°C |
| technique(s) | electrophoresis: suitable |
| Application: | Nitrotetrazolium Blue chloride has been used: • to determine the superoxide anion in skeletal muscle cells • as a component in Tris-HCl buffered saline for nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase histochemistry of brain sections • as a chromogenic substrate to study the histochemical detection of hydrogen peroxide (H2O2) and superoxide (O2- ) anion in Brassica juncea seedlings • as a component in Triton X-100 solution to perform staining of coronal brain sections |
| Biochem/physiol Actions: | Nitrotetrazolium Blue chloride (NBT) has also been used as a colorimetric indicator of bacterial infection in blood samples. NBT has also been used as a redox indicator for other enzymatic reactions. NBT produces a dark blue insoluble formazan compound while reacting with O2. |
| General description: | Nitrotetrazolium Blue chloride (NBT) is a dye that is used for detection of alkaline phosphatase in combination with 5-bromo-4-chloro-3-indoxy |
| Packaging: | 1 g in glass bottle |
| Packaging: | 50, 250 mg in glass insert |
| Symbol |  GHS02 |
| Signal word | Danger |
| Hazard statements | H242 |
| Precautionary statements | P210 - P234 - P235 - P240 - P370 + P378 - P403 |
| Hazard Codes | Xn |
| Risk Statements | 22 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Purity | >90% |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |