Nitrotetrazolium Blue chloride

SIAL/N6876 - ≥90.0% (HPLC)

Synonym: 2,2′-bis(4-Nitrophenyl)-5,5′-diphenyl-3,3′-(3,3′-dimethoxy-4,4′-diphenylene)ditetrazolium chloride; 3,3′-(3,3′-Dimethoxy-4,4′-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride]; p-Nitro-Blue tetrazolium chloride; p-Nitrotetrazolium blue; NBT; Nitro BT

CAS Number: 298-83-9
Empirical Formula (Hill Notation): C₄₀H₃₀Cl₂N₁₀O₆
Molecular Weight: 817.64
MDL Number: MFCD00012159
Linear Formula: C₄₀H₃₀N₁₀O₆ · 2Cl
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-N6876-10X10MG	10 mg	$111.00 10/EA	Add To Favorites
45-N6876-50MG	50 mg	$44.50 1/EA	Add To Favorites
45-N6876-100MG	100 mg	$68.30 1/EA	Add To Favorites
45-N6876-250MG	250 mg	$132.00 1/EA	Add To Favorites
45-N6876-500MG	500 mg	$213.00 1/EA	Add To Favorites
45-N6876-1G	1 g	$336.00 1/EA	Add To Favorites
45-N6876-5G	5 g	$1350.00 1/EA	Add To Favorites

Thoracic and abdominal segments of a Drosophila melanogaster embryo double labeled using monoclonal antibodies to engrailed (purple) and achaete (brown) proteins. Engrailed expression marks the posterior compartment of each segment and achaete expression labels five neuroblasts per hemisegment: three found midway between engrailed stripes and two found coincident with engrailed stripes. The cellular outlines of non-achaete positive neuroblasts are clearly visible interspersed among the achaete positive neuroblasts. Goat Anti-Mouse IgG-Alkaline Phosphatase conjugate (Cat. No. A7434) and BCIP/NBT (Cat. Nos. B8503/N6876) and DAB tablets (Cat. No. D5905) were used. From J. Skeath, Univ. of Wisc., Madison, WI]

This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: N6876-100MG.

Properties
Descriptions
Safety Info.
Basic Data

application(s)	diagnostic assay manufacturing hematology histology
assay	≥90.0% (HPLC)
form	powder
impurities	≤10% solvent
InChI	1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2
InChI key	FSVCQIDHPKZJSO-UHFFFAOYSA-L
Quality Level	200
SMILES string	[Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccc(cc3)[N+]([O-])=O)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccc(cc7)[N+]([O-])=O)-c8ccccc8
solubility	H₂O: 10 mg/mL
storage temp.	2-8°C

Application:	• Nitrotetrazolium Blue chloride has been used to detect the generation of oxygen metabolites in cells. • It has been used as a substrate for western blot detection. • It has been used for the staining of histological tissue sections.
Biochem/physiol Actions:	Nitrotetrazolium blue (NBT), in combination with BCIP (5-bromo-4-chloro-3-indolyl-phosphate), is required for the detection of labeled probes in western blots. It is part of the colorimetric detection assay. NBT and BCIP dyes detect alkaline phosphatase activity, thereby generating an insoluble purple dye (diformazan). The dye is visible at areas where the labeled probe has hybridized to its target.
Packaging:	1, 5 g in glass bottle
Packaging:	10×10, 50, 100, 250, 500 mg in glass insert
Recommended products:	Discover LiChropur reagents ideal for HPLC or LC-MS analysis
Substrates:	Substrate for dehydrogenases and other oxidases.

Symbol	GHS07,GHS08
Signal word	Warning
Hazard statements	H302 - H315 - H319 - H335 - H371
Precautionary statements	P260 - P264 - P301 + P312 - P302 + P352 - P305 + P351 + P338 - P308 + P311
Hazard Codes	Xn
Risk Statements	20/21/22-68/20/21/22
Safety Statements	36/37
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥90.0% (HPLC)
Storage Temp.	2-8°C
UNSPSC	12171500

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