Vitamin D metabolites in frozen human serum
SIAL/NIST972A - NIST® SRM® 972a
Product Type: Chemical
| application(s) | clinical testing |
| form | liquid |
| format | matrix material |
| grade | certified reference material |
| manufacturer/tradename | NIST® |
| packaging | pkg of 4 x 1 mL |
| Quality Level | 100  |
| technique(s) | mass spectrometry (MS): suitable |
| Application: | This vitamin D metabolite standard reference material (SRM) is intended for use as an accuracy control in the evaluation of methods for determining the amount-of-substance concentration of vitamin D metabolites in human serum. This SRM can also be used as a quality assurance tool for assigning values to in-house control materials for these constituents. |
| Application: | Vitamin D metabolites in frozen human serum has been used as a reference material for the calibration and standardization of 25-hydroxyvitamin D (25(OH)D) from dried blood spot samples by liquid chromatography-tandem mass spectrometry (LC-MS/MS). |
| Biochem/physiol Actions: | Vitamin D known as cholecalciferol exists as vitamin D3. It is taken in the diet through fortified dairy products and fish oils. It is synthesized in the skin from 7-dehydrocholesterol by ultraviolet irradiation. The biologically active form of vitamin D, 25(OH)2D3, is essential for mineral metabolism, and for other physiological functions like inhibition of growth of cancer cells and protection against certain immune mediated disorders. |
| General description: | Determining total 25-hydroxy vitamin D [25(OH)D] concentration in serum, the sum of 25-hydroxyvitamin D2 [25(OH)D2] and 25-hydroxyvitamin D3 [25(OH)D3] is generally considered a reliable indicator of vitamin D status. Measurement of 24R, 25(OH)2D3 in serum is considered a catabolism marker and an indicator of kidney disease. A unit of SRM 972a contains 1 mL of four vials (levels 1 through 4) of frozen serum with different concentration levels of 25-hydroxyvitamin D [25(OH)D] and 24R, 25-dihydroxy vitamin D3 [24R,25(OH)2D3]. NIST972A_Cert  NIST972A_SDS |
| Legal Information: | NIST is a registered trademark of National Institute of Standards and Technology |
| Legal Information: | SRM is a registered trademark of National Institute of Standards and Technology |
| Other Notes: | • Information on expiry, storage, and usage is provided in the NIST certificate. • Notes on Biomaterials, disposal, and transport are available on NIST MSDS. |
| Preparation Note: | • The vials should be stored in the dark at a temperature between –20 °C and –80 °C. • Before use, the vial/s must be thawed at room temperature for about 30 mins in subdued light. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 41116107 |