TWEEN® 20
SIAL/P1379 - viscous liquid
Synonym: Polyethylene glycol sorbitan monolaurate; Polyoxyethylenesorbitan monolaurate
CAS Number: 9005-64-5
MDL Number: MFCD00165986
Product Type: Chemical
| acid number | ≤2.2 mg/g |
| assay | ≥40.0% (GC) |
| CMC | 0.06 mM (20-25°C) |
| composition | lauric acid, ≥40% (balance primarily myristic, palmitic, and stearic acids) |
| density | 1.095 g/mL at 25 °C (lit.) |
| description | non-ionic |
| form | viscous liquid |
| HLB | 16.7 |
| hydroxyl value | 96‑108 mg/g |
| impurities | ≤3.0% water |
| InChI | 1S/C26H50O10/c1-2-3-4-5-6 |
| InChI key | HMFKFHLTUCJZJO-UHFFFAOYSA |
| mol wt | ~1228 |
| Quality Level | 300  |
| refractive index | n |
| suitability | complies for IR spectroscopy |
| technique(s) | cell culture | mammalian: suitable |
| DNA extraction: suitable | |
| protein purification: suitable | |
| RNA extraction: suitable | |
| transition temp | cloud point 76 °C |
| Application: | Non-ionic detergent |
| Application: | TWEEN 20 has been used as a component of: • washing buffer and blocking buffer in Western blotting • blocking buffer in immunohistochemistry • washing buffer in ELISA (enzyme linked immunosorbent assay) • reaction mixture in PCR (polymerase chain reaction) |
| Application: | TWEEN 20 is a nonionic detergent widely used in biochemical applications. It has been used as an emulsifying agent for the preparation of stable oil-in-water emulsions. TWEEN 20 has been used in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins). TWEEN 20 has been used as a blocking agent for membrane based immunoassays at a typical concentration of 0.05%. TWEEN 20 can be used for lysing mammalian cells at a concentration of 0.005 to 0.5%. |
| General description: | Tween®20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached. |
| Legal Information: | TWEEN is a registered trademark of Croda International PLC |
| Packaging: | 1, 4 L in poly bottle |
| Packaging: | 25, 100, 250, 500, 6×500 mL in poly bottle |
| Packaging: | P1379-1KT: Each kit contains 3 x 100ML samples, each sample from a uniquely manufactured lot. |
| Preparation Note: | TWEEN 20 is miscible in water (100mg/mL), yielding a clear, yellow solution. It is also miscible with alcohol, dioxane, and ethyl acetate. TWEEN 20 is insoluble in liquid paraffin and fixed oils. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | 527.0 °F - Pensky-Martens c |
| Flash Point(C) | 275 °C - Pensky-Martens clo |
| Purity | ≥40.0% (GC) |
| Density | 1.095 g/mL at 25 °C (lit.) |
| Refractive Index | n |
| UNSPSC | 12161900 |