TWEEN® 80
SIAL/P1754 - viscous liquid
Synonym: TWEEN® 80; POE (20) sorbitan monooleate; Polyethylene glycol sorbitan monooleate; Polyoxyethylenesorbitan monooleate; Polysorbate 80
CAS Number: 9005-65-6
MDL Number: MFCD00082107
Product Type: Chemical
| aggregation number | 60 |
| application(s) | cell analysis |
| biological source | bovine (tallow) |
| Porcine (tallow) | |
| CMC | 0.012 mM (20-25°C) |
| composition | Oleic acid, ≥58.0% (balance primarily linoleic, palmitic, and stearic acids) |
| density | 1.064 g/cm3 |
| description | non-ionic |
| form | viscous liquid |
| HLB | 15 |
| InChI | 1S/C32H60O10/c1-2-3-4-5-6 |
| InChI key | RGPBUVUVZKQNHD-MDZDMXLPSA |
| mol wt | average mol wt 1310 |
| micellar avg mol wt 79,000 | |
| Quality Level | 200  |
| SMILES string | CCCCCCCC/C=C/CCCCCCCC(=O) |
| solubility | water: soluble, clear to slightly hazy, colorless to yellow |
| transition temp | cloud point 65 °C |
| viscosity | 400-620 mPa.s |
| Application: | Non-ionic detergent used for selective protein extraction and isolation of nuclei from mammalian cell lines. |
| Application: | TWEEN® 80 has been used in drug vehicle solution for specific injections in mice and rats. It has been used for the determination of esterase activity in bacterial cells. |
| Biochem/physiol Actions: | TWEEN® 80 is a polysorbate surfactant which helps in stabilizing proteins and in protein membrane studies. It is regularly used for the extraction of membrane proteins and isolation of nuclei from cells. |
| Features and Benefits: | • Excellent wetting and dispersing agent • Highly versatile surfactant for your Cell Biology and Biochemical research |
| General description: | Tween® 80 is a non-ionic detergent and surfactant, classified as an ester of sorbitan and oleic acid. It is a versatile compound commonly employed in various biological applications, including cell lysis, nuclei isolation, and cell fractionation. Additionally, Tween® 80 finds use in stabilizing proteins and facilitating protein membrane studies. Its effectiveness stems from its membership in the polysorbate group, characterized by the combination of sorbitol and sorbitane oleic esters with polyethylene glycol chains. These structural features contribute to Tween® 80′s emulsifying capabilities and a broad range of applications. |
| Legal Information: | TWEEN is a registered trademark of Croda International PLC |
| Other Notes: | For additional information on our range of Biochemicals , please complete this form . |
| Packaging: | 1 gal in poly bottle |
| Packaging: | 1 L in poly bottle |
| Packaging: | 25, 500 mL in poly bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | 235.4 °F - closed cup |
| Flash Point(C) | > 113 °C - closed cup |
| Density | 1.064 g/cm3 |
| UNSPSC | 12161900 |