Proteinase K from Tritirachium album
SIAL/P2308 - lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology
Synonym: Endopeptidase K
CAS Number: 39450-01-6
EC Number: 254-457-8
MDL Number: MFCD00132129
Product Type: Chemical
Proteinase K illustration.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P2308-100MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P2308-1G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P2308-500MG
| composition | Protein, ≥90% biuret |
| foreign activity | DNAse, Nickase and RNAse, none detected |
| form | lyophilized powder |
| grade | for molecular biology |
| impurities | ≤1 ppm DNA |
| mol wt | 28.93 kDa |
| product line | BioUltra |
| Quality Level | 400  |
| shipped in | wet ice |
| specific activity | ≥30 units/mg protein |
| storage temp. | −20°C |
| Application: | Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA. Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A. Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria Determination of enzyme localization on membranes Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling. Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research. |
| Biochem/physiol Actions: | Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA. |
| Packaging: | 1 g in poly bottle |
| Packaging: | 10, 25, 100, 500 mg in poly bottle |
| Packaging: | 5 mg in glass bottle |
| Unit Definition: | One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 μmole of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent). |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H334 |
| Precautionary statements | P261 - P264 - P280 - P284 - P302 + P352 - P305 + P351 + P338 |
| Hazard Codes | Xn |
| Risk Statements | 36/37/38-42 |
| Safety Statements | 22-24-26-36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥30 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.4.21.64 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |