Ponceau S
SIAL/P3504 - BioReagent, suitable for electrophoresis
Synonym: 3-
CAS Number: 6226-79-5
Empirical Formula (Hill Notation): C22H12N4Na4O13S4
Molecular Weight: 760.57
EC Number: 228-319-2
MDL Number: MFCD00003892
Linear Formula: C22H12N4Na4O13S4
Product Type: Chemical
ε (extinction coefficient) | ≥330000 at 518-522 nm in deionized water at 0.015 g/L |
application(s) | diagnostic assay manufacturing hematology histology |
color | red to very dark red |
form | powder |
InChI | 1S/C22H16N4O13S4.4Na/c27- |
InChI key | KQHKSGRIBYJYFX-WNDMICSESA |
product line | BioReagent |
Quality Level | 200 |
SMILES string | [Na+].[Na+].[Na+].[Na+].O |
solubility | H2O: 1 mg/mL |
storage temp. | room temp |
technique(s) | electrophoresis: suitable |
Application: | Ponceau S has been used for membrane staining (Western blotting. |
General description: | Ponceau S is the sodium salt of a diazo dye. It is used to make a stain for rapid reversible staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. The stain is reversed with water washes and aids in subsequent immunological detection. The following are two common stain formulations: 0.1% Ponceau S (w/v) in 5% acetic acid 2% Ponceau S (w/v) in 30% TCA, 30% sulfosalicylic acid |
Packaging: | 10, 50, 100 g in glass bottle |
Hazard Codes | Xi |
Risk Statements | 36/37/38 |
Safety Statements | 26-36 |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Storage Temp. | room temp |
Colour Index Number | 27195 |
UNSPSC | 12171500 |