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Ponceau S

SIAL/P3504 - BioReagent, suitable for electrophoresis

Synonym: 3-Hydroxy-4-(2-sulfo-4-[4-sulfophenylazo]phenylazo)-2,7-naphthalenedisulfonic acid sodium salt

CAS Number: 6226-79-5
Empirical Formula (Hill Notation): C22H12N4Na4O13S4
Molecular Weight: 760.57
EC Number: 228-319-2
MDL Number: MFCD00003892
Linear Formula: C22H12N4Na4O13S4
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-P3504-10G 10 g
$52.00
1/EA
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45-P3504-50G 50 g
$160.00
1/EA
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45-P3504-100G 100 g
$238.00
1/EA
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This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P3504-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P3504-10G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P3504-50G

 

ε (extinction coefficient) ≥330000 at 518-522 nm in deionized water at 0.015 g/L
application(s) diagnostic assay manufacturing
hematology
histology
color red to very dark red
form powder
InChI 1S/C22H16N4O13S4.4Na/c27-22-20(43(37,38)39)10-12-9-16(41(31,32)33)6-7-17(12)21(22)26-25-18-8-3-14(11-19(18)42(34,35)36)24-23-13-1-4-15(5-2-13)40(28,29)30;;;;/h1-11,27H,(H,28,29,30)(H,31,32,33)(H,34,35,36)(H,37,38,39);;;;/q;4*+1/p-4/b24-23+,26-25+;;;;
InChI key KQHKSGRIBYJYFX-WNDMICSESA-J
product line BioReagent
Quality Level 200 
SMILES string [Na+].[Na+].[Na+].[Na+].Oc1c(N=Nc2ccc(cc2S([O-])(=O)=O)N=Nc3ccc(cc3)S([O-])(=O)=O)c4ccc(cc4cc1S([O-])(=O)=O)S([O-])(=O)=O
solubility H2O: 1 mg/mL
storage temp. room temp
technique(s) electrophoresis: suitable
Application: Ponceau S has been used for membrane staining (Western blotting.
General description: Ponceau S is the sodium salt of a diazo dye. It is used to make a stain for rapid reversible staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. The stain is reversed with water washes and aids in subsequent immunological detection.
The following are two common stain formulations:
0.1% Ponceau S (w/v) in 5% acetic acid
2% Ponceau S (w/v) in 30% TCA, 30% sulfosalicylic acid
Packaging: 10, 50, 100 g in glass bottle
Hazard Codes Xi
Risk Statements 36/37/38
Safety Statements 26-36
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Storage Temp. room temp
Colour Index Number 27195
UNSPSC 12171500

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