Ponceau S solution

SIAL/P7170 - BioReagent, suitable (for use in cellulose acetate electrophoresis), 0.1 % (w/v) in 5% acetic acid

Synonym: Ponceau S

CAS Number: 6226-79-5
Empirical Formula (Hill Notation): C₂₂H₁₂N₄Na₄O₁₃S₄
Molecular Weight: 760.57
MDL Number: MFCD00003892
Linear Formula: C₂₂H₁₂N₄Na₄O₁₃S₄
Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-P7170-1L	1 L	$127.00 1/EA		Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

application(s)	diagnostic assay manufacturing hematology histology
concentration	0.1 % (w/v) in 5% acetic acid
density	1.01 g/cm³ at 20 °C
form	liquid
InChI	1S/C22H16N4O13S4.4Na/c27-22-20(43(37,38)39)10-12-9-16(41(31,32)33)6-7-17(12)21(22)26-25-18-8-3-14(11-19(18)42(34,35)36)24-23-13-1-4-15(5-2-13)40(28,29)30;;;;/h1-11,27H,(H,28,29,30)(H,31,32,33)(H,34,35,36)(H,37,38,39);;;;/q;4*+1/p-4/b24-23+,26-25+;;;;
InChI key	KQHKSGRIBYJYFX-WNDMICSESA-J
product line	BioReagent
Quality Level	200
SMILES string	[Na+].[Na+].[Na+].[Na+].Oc1c(N=Nc2ccc(cc2S([O-])(=O)=O)N=Nc3ccc(cc3)S([O-])(=O)=O)c4ccc(cc4cc1S([O-])(=O)=O)S([O-])(=O)=O
storage temp.	room temp
suitability	suitable (for use in cellulose acetate electrophoresis)
	suitable for electrophoresis

Application:	Ponceau S solution has been used for membrane staining (Western blotting).
General description:	Ponceau S is the sodium salt of a diazo dye. Ponceau S solution is a suitable reagent for use in electrophoresis studies. The Ponceau S stain comes ready-to-use and is designed for rapid (5 min) staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. It may be employed as stain for the purified fibrinolytic enzyme (CK) for the analysis of N-terminal amino acid sequence of the first 14 residues of CK by a protein sequencer. Ponceau S staining on nitrocellulose and PVDF is reversible by washing the stained membrane with 0.1M NaOH for 1 minute. The stained and destained membrane can then be immunologically detected (Western blotting). A product information sheet is supplied which includes the staining procedure for cellulose acetate, nitrocellulose, and PVDF.
Packaging:	1 L in poly bottle

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Density	1.01 g/cm³ at 20 °C
Storage Temp.	room temp
UNSPSC	12171500

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