TWEEN® 20
SIAL/P7949 - BioXtra, viscous liquid
Synonym: Polyethylene glycol sorbitan monolaurate; Polyoxyethylenesorbitan monolaurate
CAS Number: 9005-64-5
MDL Number: MFCD00165986
Product Type: Chemical
anion traces | chloride (Cl-): ≤0.05% |
sulfate (SO42-): ≤0.05% | |
cation traces | Al: ≤0.0005% |
Ca: ≤0.0005% | |
Cu: ≤0.0005% | |
Fe: ≤0.0005% | |
K: ≤0.005% | |
Mg: ≤0.0005% | |
Na: ≤0.05% | |
NH4+: ≤0.05% | |
Pb: ≤0.001% | |
Zn: ≤0.0005% | |
CMC | 0.06 mM (20-25°C) |
composition | lauric acid, ≥40% (balance primarily myristic, palmoyic, and stearic acids) |
density | 1.095 g/mL at 25 °C (lit.) |
description | non-ionic |
form | viscous liquid |
HLB | 16.7 |
ign. residue | ≤0.2% |
impurities | ≤0.05% Phosphorus (P) |
InChI | 1S/C26H50O10/c1-2-3-4-5-6 |
InChI key | HMFKFHLTUCJZJO-UHFFFAOYSA |
mol wt | ~1228 |
product line | BioXtra |
Quality Level | 300 ![]() |
refractive index | n |
technique(s) | blocking: suitable |
cell culture | mammalian: suitable | |
hybridization: suitable | |
protein purification: suitable | |
transformation: suitable | |
transition temp | cloud point 76 °C |
Application: | Non-ionic detergent |
Application: | TWEEN 20 has been used in post-hybridization wash buffer after in situ hybridization. It has been used along with PBS (phosphate buffered saline) to wash wells during ELISA and along with PBS (phosphate buffered saline) in wash buffer for immunostaining. |
Application: | TWEEN 20 is a nonionic detergent widely used in biochemical applications. It has been used as an emulsifying agent for the preparation of stable oil-in-water emulsions. TWEEN 20 has been used in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins). TWEEN 20 has been used as a blocking agent for membrane based immunoassays at a typical concentration of 0.05%. TWEEN 20 can be used for lysing mammalian cells at a concentration of 0.005 to 0.5%. |
Caution: | TWEEN 20 may not be stable to autoclaving, particularly with metal cations in buffer solutions. Autoclaving is not recommended without testing for changes in properties. TWEEN 20 is heat sensitive and will darken when exposed to elevated temperatures. Polysorbates have been reported to be incompatible with alkalis, heavy metal salts, phenols, and tannic acid. They may also reduce the activity of many preservatives. Aqueous solutions of polysorbates undergo autoxidation during storage, with changes being catalyzed by light, increased temperature, and copper sulfate. |
General description: | Tween®20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached. |
Legal Information: | TWEEN is a registered trademark of Croda International PLC |
Packaging: | 100, 500 mL in poly bottle |
Preparation Note: | TWEEN 20 is miscible in water (100mg/mL), yielding a clear, yellow solution. It is also miscible with alcohol, dioxane, and ethyl acetate. TWEEN 20 is insoluble in liquid paraffin and fixed oils. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | 527.0 °F - Pensky-Martens c |
Flash Point(C) | 275 °C - Pensky-Martens clo |
Density | 1.095 g/mL at 25 °C (lit.) |
Refractive Index | n |
UNSPSC | 12161900 |