TWEEN® 20
SIAL/P8341 - Low-peroxide; Low-carbonyls
Synonym: Polyethylene glycol sorbitan monolaurate; Polyoxyethylenesorbitan monolaurate
CAS Number: 9005-64-5
MDL Number: MFCD00165986
Product Type: Chemical
| CMC | 0.06 mM (20-25°C) |
| density | 1.095 g/mL at 25 °C (lit.) |
| 1.1 g/cm3 at 25 °C (lit.) | |
| description | non-ionic |
| form | viscous liquid |
| HLB | 16.7 |
| impurities | ≤0.5 μmol/g Peroxides |
| ≤1.0 μmol/g Carbonyls | |
| ≤3.0% water | |
| InChI | 1S/C26H50O10/c1-2-3-4-5-6 |
| InChI key | HMFKFHLTUCJZJO-UHFFFAOYSA |
| mol wt | ~1228 |
| Quality Level | 200  |
| refractive index | n |
| storage temp. | −20°C |
| transition temp | cloud point 76 °C |
| Application: | Non-ionic detergent |
| Application: | TWEEN 20 is widely used in several biochemical applications. It has been used: • As an emulsifying agent for the preparation of stable oil-in-water emulsions • In pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins) • As a blocking agent for membrane based immunoassays at a typical concentration of 0.05% • For lysing mammalian cells at a concentration of 0.005 to 0.5% |
| General description: | Tween®20, is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached. |
| Legal Information: | TWEEN is a registered trademark of Croda International PLC |
| Other Notes: | Preservative Free |
| Packaging: | 10, 5×10 mL in glass bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Density | 1.095 g/mL at 25 °C (lit.); 1.1 g/cm3 at 25 °C (lit.) |
| Refractive Index | n |
| Storage Temp. | −20°C |
| UNSPSC | 12161900 |