Methotrexate
SIAL/PHR1396 - Pharmaceutical Secondary Standard; Certified Reference Material
Synonym: Methotrexate; (+)-Amethopterin; 4-
CAS Number: 59-05-2
Empirical Formula (Hill Notation): C20H22N8O5
Molecular Weight: 454.44
EC Number: 200-413-8
MDL Number: MFCD00064370
Linear Formula: C20H22N8O5
Product Type: Chemical
agency | traceable to Ph. Eur. M1000000 |
traceable to USP 1414003 | |
application(s) | pharmaceutical (small molecule) |
CofA | current certificate can be downloaded |
format | neat |
grade | certified reference material |
pharmaceutical secondary standard | |
InChI | 1S/C20H22N8O5/c1-28(9-11- |
InChI key | FBOZXECLQNJBKD-ZDUSSCGKSA |
Quality Level | 300 ![]() |
SMILES string | CN(Cc1cnc2nc(N)nc(N)c2n1) |
storage temp. | 2-8°C |
technique(s) | gas chromatography (GC): suitable |
HPLC: suitable |
Analysis Note: | These secondary standards offer multi-traceability to the USP, EP (PhEur) and BP primary standards, where they are available. |
Application: | Methotrexate may be used as a pharmaceutical secondary reference standard for the determination of the analyte in plasma samples and pharmaceutical formulations by various techniques. |
Application: | These Secondary Standards are qualified as Certified Reference Materials. These are suitable for use in several analytical applications including but not limited to pharma release testing, pharma method development for qualitative and quantitative analyses, food and beverage quality control testing, and other calibration requirements. |
Footnote: | To see an example of a Certificate of Analysis for this material enter LRAB9958 in the slot below. This is an example certificate only and may not be the lot that you receive. |
General description: | Pharmaceutical secondary standards for application in quality control, provide pharma laboratories and manufacturers with a convenient and cost-effective alternative to the preparation of in-house working standards. Methotrexate is an antagonist of folic acid that inhibits dihydrofolate reductase (DHFR) enzyme, reversibly and results in impaired DNA synthesis and cellular replication. Methotrexate has been used in treatment of rheumatoid arthritis and also for maintenance therapy in childhood acute lymphocytic leukemia (ALL). |
Other Notes: | This Certified Reference Material (CRM) is produced and certified in accordance with ISO 17034 and ISO/IEC 17025. All information regarding the use of this CRM can be found on the certificate of analysis. |
Symbol | ![]() ![]() |
Signal word | Danger |
Hazard statements | H301 - H315 - H319 - H341 - H360D |
Precautionary statements | P201 - P301 + P310 + P330 - P302 + P352 - P305 + P351 + P338 |
Hazard Codes | T |
Risk Statements | 46-61-25-36/38 |
Safety Statements | 53-26-36-45 |
RIDADR | UN 1544PSN1 6.1 / PGIII |
WGK Germany | WGK 3 |
Storage Temp. | 2-8°C |
UNSPSC | 41116107 |