Tris Buffered Saline
SIAL/PPB001 - pH 8.0, pHast Pack™, powder
Synonym: 1X TBS wash buffer; TBS
MDL Number: MFCD00132476
Product Type: Chemical
foreign activity | DNAse, none detected |
Nickase, none detected | |
Protease, none detected | |
RNAse, none detected | |
form | powder |
pH | 8.0 |
product line | pHast Pack™ |
Application: | Tris-buffered saline is intended to use for: • Sample preparation • ELISA • Western Blot and other protein applications • Cell culture (recommend to sterile filter) • Immunohistochemistry • Resuspension and sonication of cells |
Features and Benefits: | • Ready-to-use buffer – fast and easy to prep • No need to measure or adjust pH, simply add water • Saves time and minimizes the effort • Biological tests: free of DNase, RNase, protease, and nickase • Chemical tests: Iron ≤10 ppm, lead ≤5 ppm |
General description: | Tris Buffered Saline, pH 8.0 is a commonly used wash buffer that is isotonic and nontoxic and suitable for use in cell culture and electrophoresis applications. |
Legal Information: | pHast Pack is a trademark of Sigma-Aldrich Co. LLC |
Packaging: | Foil pouches |
Reconstitution: | Contents of one pouch, when dissolved in 500mL of distilled or deionized water, will yield a 1X solution containing 50mM Tris, 138mM NaCl, 2.7mM KCl, pH 8.0 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free. Certified for use in cell culture. |
Storage and Stability: | Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use. |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
UNSPSC | 12161700 |