Tris Buffered Saline, with Tween® 20, pH 8.0
SIAL/PPB002 - powder, pHast Pack™
Product Type: Chemical
form | powder |
product line | pHast Pack™ |
Application: | Suitable for use as a wash buffer: • Western blot • ELISA • Immunohistochemistry |
Features and Benefits: | • Ready-to-use buffer • No measuring and pH adjusting needed • Save time and minimize effort • Biological tests: free of DNase, RNase, protease, and nickase • Chemical tests: Iron ≤10 ppm, lead ≤5 ppm |
General description: | Tris Buffered Saline with 0.05% Tween® 20 or TBST has a low concentration of detergent with a pH of 8.0. It is a common washing buffer used in many immunodetection techniques. It decreases non-specific bindings from the western blot membrane and microtiter plate walls. |
Legal Information: | pHast Pack is a trademark of Sigma-Aldrich Co. LLC |
Legal Information: | TWEEN is a registered trademark of Croda International PLC |
Packaging: | Foil pouches |
Reconstitution: | Contents of one pouch, when dissolved in 500 mL of distilled or deionized water, will yield a 1× solution containing 50 mM Tris, 138 mM NaCl, 2.7 mM KCl; 0.05%Tween 20 (w/v), pH 8.0 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free. |
Storage and Stability: | Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use. |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |