Phosphate buffered saline
SIAL/PPB006 - pH 7.4, pHast Pack™, powder
Synonym: 1X PBS wash buffer; 1X PBS wash buffer; PBS
MDL Number: MFCD00131855
Product Type: Chemical
pHast pack pouches displayed with box
Gloved hand holding pHast pack pouch
| foreign activity | DNAse, none detected |
| Nickase, none detected | |
| Protease, none detected | |
| RNAse, none detected | |
| form | powder |
| pH | 7.4 |
| product line | pHast Pack™ |
| Application: | Phosphate buffered saline is commonly used in washing solutions for: • ELISA • Western Blotting and other protein applications • Cell culture (sterile filtration recommended) • Resuspension and sonication of cells • Immunohistochemistry |
| Features and Benefits: | • Save time with our ready-to-use formulation • No calculations, measurements, and adjusting pH • Reliable quality for reproducibility • Suitable for use in cell culture and electrophoresis • Biological tests: free of DNase, RNase, Protease, and Nickase • Chemical tests: Iron ≤10 ppm, Lead ≤5 ppm |
| General description: | Phosphate buffered saline or PBS, pH 7.4 is a commonly used biological buffer in molecular biology. A 1X working solution is typically used in cell culture and electrophoresis assays to wash away non-adherent cells or any unbound biomolecules. PBS is hypo-osmotic and can be harmful to cells, causing swelling and resulting in cell lysis, if left for extended periods. |
| Legal Information: | pHast Pack is a trademark of Sigma-Aldrich Co. LLC |
| Packaging: | Foil pouches |
| Reconstitution: | Contents of one pouch, when dissolved in 500mL of distilled or deionized water, will yield a 1X solution containing 10mM Na2HPO4, 1.8mM KH2PO4, 138mM NaCl, 2.7mM KCl, pH 7.4 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free. Certified for use in cell culture. |
| Storage and Stability: | Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 12161700 |