Tris Acetate-EDTA buffer
SIAL/PPB008 - pH 8.3, pHast Pack™, powder
Synonym: 1X TAE buffer; Tris Acetate EDTA; TAE buffer
MDL Number: MFCD00236357
Product Type: Chemical
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| foreign activity | DNAse, none detected |
| Nickase, none detected | |
| Protease, none detected | |
| RNAse, none detected | |
| form | powder |
| InChI | 1S/C10H16N2O8.C4H11NO3.C2 |
| InChI key | HGEVZDLYZYVYHD-UHFFFAOYSA |
| pH | 8.3 |
| product line | pHast Pack™ |
| SMILES string | CC(O)=O.NC(CO)(CO)CO.OC(= |
| suitability | suitable for gel electrophoresis (after dilution to working concentration) |
| Application: | Suitable as a running and gel preparation buffer in: • DNA agarose gel electrophoresis • Non-denaturing RNA agarose gel electrophoresis for RNA >1500 bases • Denaturing gradient gel electrophoresis • Northern and Southern blotting |
| Application: | TAE running buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis. Double-stranded DNA tends to run faster in TAE than in other buffers but can also become exhausted during extended electrophoresis. Buffer circulation or buffer replacement during extended electrophoresis can remedy the lower buffering capacity. Dilution of the concentrated TAE buffer produces a 1× TAE buffer with 40 mM Tris-acetate and 1 mM EDTA, pH 8.3. The 1× TAE buffer is used both in the agarose gel and as a running buffer. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution. |
| Features and Benefits: | • Ready for use – saves time and effort • No measuring and pH adjusting needed • Eliminate exposure to toxic chemicals to prepare buffers • Biological tests: free of DNase, RNase, Protease, and Nickase • Chemical tests: Iron ≤10 ppm, lead ≤5 ppm |
| General description: | Tris Acetate-EDTA (TAE) buffer, with a pH range of 8.1 – 8.4, is typically used for agarose gel electrophoresis, especially for the recovery of DNA. For larger double-stranded DNA fragments (>12kB) TAE buffer is preferred. However, the buffering capacity can become exhausted if electrophoresis time is extended. Buffer circulation or replacement during electrophoresis can remedy the lower buffering capacity. |
| Legal Information: | pHast Pack is a trademark of Sigma-Aldrich Co. LLC |
| Packaging: | Foil pouches |
| Preparation Note: | Prepared with Biotechnology Performance Certified Trizma base (T6066) and Molecular Biology Reagent EDTA disodium salt (E5134). Solutions also contain acetic acid (A6283); powdered blend contains Trizma acetate (T1258). |
| Reconstitution: | Contents of one pouch, when dissolved in 500mL of distilled or deionized water, will yield a 1X solution containing 40mM Tris-Acetate, 1mM EDTA, pH 8.3 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free. Certified for use in electrophoresis. |
| Storage and Stability: | Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use. |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 12352200 |