1.0% Agarose in Tris-Acetate EDTA Buffer
SIAL/PPB012 - pHast Pack™, powder
Synonym: 1% Agarose gel; TAE Agarose blend
Product Type: Chemical
pHast pack pouches displayed with box
Gloved hand holding pHast pack pouch
| form | powder |
| pH | 8.1-8.5(range for the Tris Acetate ETDA buffer prior to blending with agarose) |
| product line | pHast Pack™ |
| Quality Level | 100  |
| Application: | 1% Agarose in 1X TAE can be used in:• DNA Electrophoresis • Native RNA Electrophoresis |
| Features and Benefits: | • Quick ready-to-pour 1% agarose gel – prepares 20 gels • No buffer prep or weighing needed • Save time, and effort, and minimize costs, compared to precast gels • Biological tests: free of DNase, RNase, Protease, and Nickase • Chemical tests: Iron ≤10 ppm, lead ≤5 ppm • Compatible with TAE pHast pack™ buffer |
| General description: | 1% Agarose in Tris Acetate EDTA buffer is a powder blend that readily dissolves in boiling water to quickly make an agarose gel for electrophoresis. 1% Agarose gel can separate DNA or RNA fragments that range from 400 – 8,000 bp. Typically used for electrophoresis of larger nucleic acid fragments. |
| Legal Information: | pHast Pack is a trademark of Sigma-Aldrich Co. LLC |
| Packaging: | Foil pouches |
| Preparation Note: | Prepares 250mL for casting several standard-size gels or one large agarose gel. A small electrophoresis apparatus maybe use 30 - 50mL, medium 100mL, and large rigs 250mL. Add nucleic acid stain before pouring the gel and for optimal results pair with pHast pack 1X TAE, pH 7.3 running buffer. |
| Reconstitution: | Contents of one pouch, when dissolved in 250 mL of ultrapure water, will yield a 1× solution containing 40 mM Tris acetate, 1 mM EDTA, and 1.0% (w/v) agarose. Contents tested to be DNAse, RNAse, and Nickase free. Prepare in a 1L flask. Combine pHast pack contents with 250 mL ultrapure water and mix well. Microwave on high for ~1min to boil and dissolve the agarose. Use a heat resistant glove to remove the flask every 10 - 15s to mix gently by swirling the liquid in a circle at the bottom of the flask. Repeat until the agarose is fully dissolved. Cool the flask slightly with cold water while swirling every 10 – 15s, add the stain and pour the gel. Allow agarose gel to fully cool and solidify before removing combs. |
| Storage and Stability: | Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use. |
| WGK Germany | WGK 3 |