1.0% Agarose in Tris-Borate EDTA Buffer
SIAL/PPB013 - pHast Pack™, powder
Synonym: 1% Agarose gel; TAE Agarose blend; TBE Agarose blend
Product Type: Chemical
pHast pack pouches displayed with box
Gloved hand holding pHast pack pouch
| form | powder |
| pH | 8.1-8.5(range for the Tris Borate ETDA buffer prior to blending with agarose) |
| product line | pHast Pack™ |
| Quality Level | 100  |
| Application: | 1% Agarose in TBE can be used in:• DNA Electrophoresis • Native RNA Electrophoresis |
| Features and Benefits: | • Quick ready-to-pour 1% agarose gel • No buffer preparation or weighing is needed • Save time and minimize cost and effort • Biological tests: free of DNase, RNase, Protease, and Nickase • Chemical tests: Iron ≤10 ppm, Lead ≤5 ppm |
| General description: | 1% Agarose in Tris Borate EDTA is a powder blend that can be dissolved in boiling water to quickly make a gel for electrophoresis. 1% Agarose gel can separate DNA or RNA fragments between a range of 300 – 7,000 bp and is ideal for smaller fragments <1kB. |
| Legal Information: | pHast Pack is a trademark of Sigma-Aldrich Co. LLC |
| Packaging: | Foil pouches |
| Preparation Note: | Each packet prepares 250mL of molten agarose that is typically used to cast horizontal gels. A small electrophoresis apparatus may use 30 - 50mL, medium 100mL, and large rigs 250mL. Add nucleic acid stain before pouring the gel. For optimal results pair with pHast pack running buffer 1X TBE, pH 7.3. |
| Reconstitution: | Contents of one pouch, when dissolved in 250 mL of ultrapure water, will yield a 1× solution containing 89 mM Tris borate, 2 mM EDTA, and 1.0% (w/v) agarose. Certified to be DNAse, RNAse, and Nickase free. Prepare in a 1L flask. Combine pHast pack contents with 250 mL ultrapure water and mix well. Microwave on high for ~1min to boil and dissolve the agarose. Use a heat resistant glove to remove the flask every 10 - 15s to mix gently by swirling the liquid in a circle at the bottom of the flask. Repeat until the agarose is fully dissolved. Cool the flask slightly with cold water while swirling every 10 – 15s, add the stain and pour the gel. Allow agarose gel to fully cool and solidify before removing combs. |
| Storage and Stability: | Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H360FD |
| Precautionary statements | P201 - P202 - P280 - P308 + P313 - P405 - P501 |
| WGK Germany | WGK 3 |