Bis-Tris Bicine EDTA buffer
SIAL/PPB019 - pH 7.2, pHast Pack™, powder
Synonym: 1X Bis-Tris transfer buffer; 1X Bis-Tris transfer buffer
Product Type: Chemical
form | powder |
pH | 7.2 |
product line | pHast Pack™ |
Quality Level | 100 ![]() |
Application: | Intended for use in western blotting as a transfer buffer. |
Features and Benefits: | • Ready-to-use buffer • No measuring and pH adjustment needed • Certified quality for reproducibility • Minimizes exposure to toxic chemicals • Biological tests: free of DNase, RNase, Protease, and Nickase • Chemical tests: Iron ≤10 ppm, Lead ≤5 ppm |
General description: | Bis-Tris Bicine EDTA buffer contains Bis-Tris and Bicine, zwitterionic buffer components, which can form strong bonds with common metals along with ethylenediaminetetraaceti |
Legal Information: | pHast Pack is a trademark of Sigma-Aldrich Co. LLC |
Packaging: | Foil pouches |
Reconstitution: | Contents of one pouch, when dissolved in 500 mL of ultrapure water, will yield a 1X solution containing 25 mM Bicine, 25 mM Bis-Tris, 1 mM EDTA, pH 7.2 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free. |
Storage and Stability: | Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use. |
Symbol | ![]() |
Signal word | Danger |
Hazard statements | H318 |
Precautionary statements | P280 - P305 + P351 + P338 |
WGK Germany | WGK 3 |