SSPE Buffer
SIAL/PPB021 - pH 7.4, pHast Pack™, powder
Synonym: 1X SSPE Hybridization buffer; 1X SSPE buffer; SSPE Hybridization buffer; Saline-Sodium Phosphate-EDTA Buffer
Product Type: Chemical
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pHast pack pouches displayed with box
| form | powder |
| pH | 7.4 |
| product line | pHast Pack™ |
| Quality Level | 100  |
| Application: | Saline-Sodium Phosphate-EDTA is used as a hybridization buffer in Northern and Southern blotting. It is also used as a wash buffer in DNA-microarray technique. |
| Legal Information: | pHast Pack is a trademark of Sigma-Aldrich Co. LLC |
| Packaging: | Foil pouches |
| Reconstitution: | Contents of one pouch, when dissolved in 1000 mL of ultrapure water, will yield a 1× solution containing 150 mM NaCl, 10 mM NaH2PO4, 1 mM EDTA, pH 7.4 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free. |
| Storage and Stability: | Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use. |
| WGK Germany | WGK 1 |