Tris buffer
SIAL/PPB022 - Pre-set, pH 7.0, pHast Pack™, powder
Synonym: 0.1M Tris
Product Type: Chemical
form | powder |
pH | 7.0 |
product line | pHast Pack™ |
Quality Level | 100 ![]() |
Application: | Tris buffer is used in: • Western blot • Protein gel electrophoresis • cell culture (sterile filter buffer) • As a buffer component |
Features and Benefits: | • Fast, ready-to-use buffer • No need to measure and adjust the pH • Saves time and minimizes effort in the lab • Biological tests: free of DNase, RNase, Protease, and Nickase • Chemical tests: Iron ≤10 ppm, lead ≤5 ppm |
General description: | Tris buffer, pH 7.0 is a general buffer that is isotonic and non-toxic in nature due to the presence of tris base. It is suitable for most biochemical and molecular biology applications. Tris has a buffering capacity range of pH 7.0 - 9.0, with a pKa of 8.1 at 25°C. |
Legal Information: | pHast Pack is a trademark of Sigma-Aldrich Co. LLC |
Packaging: | Foil pouches |
Reconstitution: | Contents of one pouch, when dissolved in 500 mL of ultrapure water, will yield a 0.1M Tris solution, pH 7.0 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free. This product is suitable for use in cell culture and electrophoresis applications. |
Storage and Stability: | Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use. |
WGK Germany | WGK 1 |