Imidazole
SIAL/RDD039 - for molecular biology, ≥99% (titration), free-flowing, Redi-Dri™
Synonym: 1,3-
CAS Number: 288-32-4
Empirical Formula (Hill Notation): C3H4N2
Molecular Weight: 68.08
MDL Number: MFCD00005183
Linear Formula: C3H4N2
Product Type: Chemical
| application(s) | diagnostic assay manufacturing |
| assay | ≥99% (titration) |
| bp | 256 °C (lit.) |
| foreign activity | DNase, RNase and protease, none detected |
| form | crystalline powder |
| grade | for molecular biology |
| InChI | 1S/C3H4N2/c1-2-5-3-4-1/h1 |
| InChI key | RAXXELZNTBOGNW-UHFFFAOYSA |
| mp | 88-91 °C (lit.) |
| pH | 6.2-7.8 (25 °C) |
| pKa (25 °C) | 6.95 |
| product line | Redi-Dri™ |
| quality | free-flowing |
| Quality Level | 100  |
| SMILES string | c1c[nH]cn1 |
| vapor pressure | <1 mmHg ( 20 °C) |
| Application: | Excellent for buffers in the range of pH 6.2-7.8 |
| Application: | Imidazole has been used • in the lysis, wash and elution buffer for the purification of histidine tagged Sonic Hedgehog(shh-N) protein • in elution buffer in stepwise gradient for the purification of histidine tagged aldo keto reductases using nickel affinity chromatography • as a component of homogenization buffer for the purification of phagosomal compartments from dendritic cells |
| Biochem/physiol Actions: | Imidazole derivatives have antibacterial, antifungal and anticancer functionality. It interacts with DNA and also binds to protein and stops cell division. It also acts as a microtubule destabilizing agents and inhibits topoisomerase and Cytochrome P450 Family 26 Subfamily A Member 1 (CYP26A1) enzymes. Imidazole based anticancer drug find applications in cancer chemotherapy. It is used as buffer component for purification of the histidine tagged recombinant proteins in immobilized metal-affinity chromatography (IMAC). |
| General description: | Imidazole is a heterocyclic compound with a five-membered planar ring. It is amphoteric and highly polar. The pharmacophore of imidazole exists in bioactive compounds including amino acids, plant growth regulators and therapeutic agents. |
| Legal Information: | Redi-Dri is a trademark of Sigma-Aldrich Co. LLC |
| Symbol |    GHS05,GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H302 - H314 - H360D |
| Precautionary statements | P201 - P260 - P280 - P303 + P361 + P353 - P305 + P351 + P338 + P310 - P308 + P313 |
| WGK Germany | WGK 2 |
| Flash Point(F) | 293.0 °F - closed cup |
| Flash Point(C) | 145 °C - closed cup |
| Purity | ≥99% (titration) |
| bp | 256 °C (lit.) |
| mp | 88-91 °C (lit.) |
| UNSPSC | 12352200 |