Safranin O
SIAL/S2255 - Dye content ≥85 %
Synonym: Basic Red 2; Cotton Red; Gossypimine; Safranin T; Safranin Y or A
CAS Number: 477-73-6
Empirical Formula (Hill Notation): C20H19ClN4
Molecular Weight: 350.84
EC Number: 207-518-8
MDL Number: MFCD00011759
Linear Formula: C20H19ClN4
Product Type: Chemical
| εmax | 1250-1650 at 530-534 nm in 50% ethanol |
| application(s) | diagnostic assay manufacturing hematology histology |
| composition | Dye content, ≥85% |
| form | powder |
| InChI | 1S/C20H18N4.ClH/c1-12-8-1 |
| InChI key | OARRHUQTFTUEOS-UHFFFAOYSA |
| pH | 10 (20 °C, 10 g/L) |
| Quality Level | 200 ![]() |
| SMILES string | [Cl-].Cc1cc2nc3cc(C)c(N)c |
| solubility | water: 1 mg/mL, clear, dark red to very dark red and red purple |
| storage temp. | room temp |
| technique(s) | microbe id | staining: suitable |
| Application: | Safranin O has been used in the histological analysis of femurs. It has also been used to detect the presence of glycosaminoglycans in the inner body of meniscus structures. |
| General description: | Safranin O is a cationic dye, which displays less significant metachromasia. It is used to detect any variations in articular disease. Safranin O binds specifically to polyanions. It detects the amount of proteoglycan present in cartilages. Safranin O is used as a marker for glycosaminoglycan depletion in osteoarthritis. Safranin is used as a counter stain in Gram staining. |
| Packaging: | 25, 100 g in glass bottle |
| Symbol | GHS05 |
| Signal word | Danger |
| Hazard statements | H318 |
| Precautionary statements | P280 - P305 + P351 + P338 + P310 |
| Hazard Codes | Xi |
| Risk Statements | 41 |
| Safety Statements | 26-39 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Storage Temp. | room temp |
| Colour Index Number | 50240 |
| UNSPSC | 12171500 |


