Spirit blue agar
SIAL/S4306 - suitable for microbiology, NutriSelect® Plus
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: S4306-500G
| application(s) | bioburden testing cleaning products cosmetics food and beverages personal care |
| microbiology | |
| final pH | 6.8±0.2 (25 °C) |
| form | powder |
| manufacturer/tradename | NutriSelect® Plus |
| product line | BioChemika |
| Quality Level | 200  |
| shelf life | limited shelf life, expiry date on the label |
| sterility | non-sterile |
| suitability | lipolytic bacteria |
| nonselective and differential for Staphylococcus spp. | |
| technique(s) | microbe id | specific enzyme detection: suitable |
| microbiological culture: suitable |
| Application: | Used for the detection and enumeration of lipolytic microogranisms. |
| Components: | Ingredients (g/L) Casein enzymic hydrolysate, 10.00 Yeast extract, 5.00 Spirit blue, 0.15 Agar, 17.00 |
| Footnote: | We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance. |
| Legal Information: | GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Preparation Note: | Suspend 32.15 g of Spirit Blue Agar in 1000 ml of distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Cool to 50°C and add slowly 30 ml of the lipase substrate while agitating to obtain an even distribution. Note: To prepare the lipase substrate mix 1 ml of Tween 80 (Cat. No. 93780) in 400 ml warm distilled water. Add 100 ml of Cottonseed Oil (Cat. No. C7767) or Olive Oil (Cat. No. 75343). Agitate vigorously to emulsify. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 41171606 |