Schiff’s fuchsin-sulfite reagent
SIAL/S5133 - suitable for detection of glycoproteins
Synonym: Schiff reagent
Empirical Formula (Hill Notation): C20H21N3O3S
Molecular Weight: 383.46
Linear Formula: C20H21N3O3S
Product Type: Chemical
| application(s) | diagnostic assay manufacturing hematology histology |
| assay | 3-5 mM iodine (I) basis (titration) |
| color | colorless |
| density | 0.990 g/cm3 |
| form | liquid |
| Quality Level | 100  |
| solubility | water: soluble |
| storage temp. | 2-8°C |
| suitability | suitable for detection of glycoproteins |
| Application: | Schiff′s fuchsin-sulfite reagent has been used: • in periodic acid-Schiff (PAS) staining to determine goblet cells and salivary glycopreoteins like mucins • to stain ovaries and testis • to stain tomato and Medicago primary roots • to stain undifferentiated sarcomas (USARCs) It provides a system to easily detect the sugar moieties of glycoproteins on SDS PAGE or Western Blotting membranes. |
| General description: | Schiff’s fuchsin-sulfite reagent is a part of the glycoprotein detection kit. It is primarily used in the Periodic Acid-Schiff (PAS) staining method to detect and visualize aldehydes, glycoproteins in biological samples. The reagent consists of basic fuchsin dye and sodium bisulfite. It reacts with the aldehyde groups of carbohydrates and produces a pink to magenta color, allowing for the identification of these substances in samples. |
| Packaging: | 1 L in poly bottle |
| Packaging: | 500 mL in poly bottle |
| Preparation Note: | Prepared using pararosaniline. |
| Symbol |   GHS05,GHS08 |
| Signal word | Danger |
| Hazard statements | H290 - H350 |
| Precautionary statements | P202 - P234 - P280 - P308 + P313 - P390 - P405 |
| RIDADR | UN 1789 8 / PGIII |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | 3-5 mM iodine (I) basis (titration) |
| Density | 0.990 g/cm3 |
| Storage Temp. | 2-8°C |
| UNSPSC | 12171500 |