SYBR® Green I nucleic acid gel stain
SIAL/S9430 - 10,000 × in DMSO
Synonym: DNA stain; SYBR® green gel dye; safer gel stain
CAS Number: 163795-75-3
MDL Number: MFCD00284715
Product Type: Chemical
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
| concentration | 10,000 × in DMSO |
| form | solution |
| greener alternative category | Aligned  , |
| greener alternative product characteristics | Designing Safer Chemicals Learn more about the Principles of Green Chemistry . |
| Quality Level | 200 |
| storage temp. | −20°C |
| sustainability | Greener Alternative Product |
| technique(s) | PCR: suitable |
| usage | mL sufficient for 100 mini-gels |
| Application: | SYBR® Green I nucleic acid gel stain has been used for: • the quantification of dsDNA • to stain DNA in polymerase chain reaction (PCR) • for comet assay technique • to assess spermatozoon membrane integrity • for visual inspection of DNA amplified by loop-mediated isothermal amplification (LAMP) • as a fluorescent dye in flow cytometry • real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) for staining reverse-transcribed cDNA |
| Features and Benefits: | • An ultrasensitive stain for post-electrophoresis staining of dsDNA in agarose or polyacrylamide gels • It can also detect ssDNA and RNA in denaturing agarose/formaldehyde and polyacrylamide/urea gels without any pre-washing steps • It is less mutagenic than ethidium bromide in Ames tests • It provides 50-100 times greater detection sensitivity than ethidium bromide for oligonucleotides • Useful for many applications with a limited amount of DNA • The binding of SYBR® Green I to DNA does not inhibit the activity of many common restriction endonucleases, including Hind III and EcoR I • Removal of this stain in-gel digestion and ligation techniques is not needed • SYBR Green I is a greener alternative product to ethidium bromide for staining |
| General description: | SYBR® Green I is a proprietary asymmetrical cyanine dye, which is used to detect nucleic acids. It consists of a N-alkylated benzothiazolium or benzoxazolium ring system, that is joined by a monomethine bridge to a pyridinium or quinolinium ring system. SYBR Green I binds to the minor groove of dsDNA and is excited at a wavelength of 480 nm. It has a peak fluorescence emission of 520 nm. |
| Legal Information: | SYBR is a registered trademark of Thermo Fisher Scientific or its subsidiaries |
| Preparation Note: | Store the product at –20 °C. The diluted Staining Solution may be stored, protected from light, either at 2–8 °C for several weeks or at room temperature for 3–4 days. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | 201.2 °F - closed cup |
| Flash Point(C) | 94 °C - closed cup |
| Storage Temp. | −20°C |
| UNSPSC | 12171500 |