SYBR® Green I nucleic acid gel stain
SIAL/S9430 - 10,000 × in DMSO
Synonym: DNA stain; SYBR® green gel dye; safer gel stain
CAS Number: 163795-75-3
MDL Number: MFCD00284715
Product Type: Chemical
concentration | 10,000 × in DMSO |
greener alternative category | Aligned ![]() |
greener alternative product characteristics | Designing Safer Chemicals Learn more about the Principles of Green Chemistry ![]() |
Quality Level | 200 ![]() |
storage temp. | −20°C |
usage | mL sufficient for 100 mini-gels |
Application: | SYBR® Green I nucleic acid gel stain has been used for: • the quantification of dsDNA • to stain DNA in polymerase chain reaction (PCR) • for comet assay technique • to assess spermatozoon membrane integrity • for visual inspection of DNA amplified by loop-mediated isothermal amplification (LAMP) • as a fluorescent dye in flow cytometry • real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) for staining reverse-transcribed cDNA |
Features and Benefits: | • An ultrasensitive stain for post-electrophoresis staining of dsDNA in agarose or polyacrylamide gels • It can also detect ssDNA and RNA in denaturing agarose/formaldehyde and polyacrylamide/urea gels without any pre-washing steps • It is less mutagenic than ethidium bromide in Ames tests • It provides 50-100 times greater detection sensitivity than ethidium bromide for oligonucleotides • Useful for many applications with a limited amount of DNA • The binding of SYBR® Green I to DNA does not inhibit the activity of many common restriction endonucleases, including Hind III and EcoR I • Removal of this stain in-gel digestion and ligation techniques is not needed • SYBR Green I is a greener alternative product to ethidium bromide for staining |
General description: | SYBR® Green I is a proprietary asymmetrical cyanine dye, which is used to detect nucleic acids. It consists of a N-alkylated benzothiazolium or benzoxazolium ring system, that is joined by a monomethine bridge to a pyridinium or quinolinium ring system. SYBR Green I binds to the minor groove of dsDNA and is excited at a wavelength of 480 nm. It has a peak fluorescence emission of 520 nm. |
Legal Information: | SYBR is a registered trademark of Life Technologies |
Storage and Stability: | Store the product at –20 °C. The diluted Staining Solution may be stored, protected from light, either at 2–8 °C for several weeks or at room temperature for 3–4 days. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | 201.2 °F - closed cup |
Flash Point(C) | 94 °C - closed cup |
Storage Temp. | −20°C |
UNSPSC | 12171500 |