FITC labeled vancomycin
SIAL/SBR00028 - for fluorescent microbial imaging
Synonym: Vancomycin -FITC; Vancomycin FITC conjugate
Empirical Formula (Hill Notation): C87H88Cl2N10O29S
Molecular Weight: 1840.65
Linear Formula: C87H88Cl2N10O29S
Product Type: Chemical
Bacillus cereus cells stained with FITC labeled Vancomycin B. cereus cells were harvested at mid-log phase. Pellets were washed with PBSX1 and incubated at a final concentration of 25 μg/mL FITC labeled Vancomycin solution at room temperature for ~10 min, washed twice with PBSX1. Images were acquired immediately after staining using Olympus BX53F2 upright microscope (X40 magnification). Image depicts FITC labeled Vancomycin staining, excitation/emission wavelength at 495 nm/515 nm.
| availability | not available in China |
| form | solid |
| mol wt | Mw 1840.66 g/mol |
| Quality Level | 100  |
| shipped in | dry ice |
| storage temp. | −20°C |
| suitability | suitable for (Fluorescent detection of gram-positive bacteria) |
| suitable for (Microbiome) | |
| suitable for microbiology |
| Analysis Note: | • Aliquots of the DMSO solution can be stored at -20°C, protected from light for at least 1 month. • Fluorescent microscopy application: Vancomycin -FITC has excitation/emission wavelength at 495 nm/515 nm. • It is recommended to dissolve Vancomycin-FITC in DMSO (Dimethyl Sulfoxide) to a concentration of 1mg/mL. • Recommended working concentration for Bacillus cereus in fluorescent microscopy imaging application is 25μg/mL. |
| Application: | Fluorescent antibiotics can be used for many applications including: • Antimicrobial resistance research. • Bacterial visualization and imaging. • Parent antibiotic mode of action research and new antibiotic discovery. • Toxicity studies. • Research of bacterial infections and tracking its uptake in vivo. |
| Biochem/physiol Actions: | Vancomycin inhibits cell wall synthesis at the level of peptidoglycan biosynthesis. Vancomycin binds with high affinity and specificity to the terminal D-Ala-D-Ala of the growing peptide chain during cell wall synthesis. The binding inhibits transpeptidase activity, which prevents further elongation and cross-linking of the peptidoglycan matrix. Vancomycin-FITC has fluorescein attached to the amine group on the disaccharide moiety of the vancomycin molecule. This disaccharide moiety does not play any role in D-Ala-D-Ala binding site, affording a comparable binding affinity of Vancomycin-FITC to Gram-positive bacteria as the parent drug. However, a minimum inhibitory concentration (MIC) decrease of about two orders of magnitude in comparison to Vancomycin was observed. This activity decrease can be linked to repulsive interactions between the negatively charged Fluorescein moiety and the anionic environment of the peptidoglycan. |
| General description: | FITC labeled Vancomycin (Vancomycin-FITC), is a Fluorescein conjugated Vancomycin, with a similar mode of action as Vancomycin. Vancomycin -FITC can be used to detect Gram-positive bacteria by common fluorescent instruments. Vancomycin is a glycopeptide antibiotic, used to treat gram-positive infections. Fluorescent antibiotics are obtained by synthetic conjugation of an antibiotic to a fluorophore. |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |