Dansyl labeled polymyxin B Ready Made Solution
SIAL/SBR00029 - for fluorescent microbial imaging, 1.5 mg/mL in H2O
Synonym: DSL-PMB; DSL-PMX; DSL-PxB
Product Type: Chemical
Escherichia coli MG1655 cells stained with Dansyl Polymyxin B (DSL-PMB) E. coli MG1655 cells were harvest at mid-log phase. Pellets were washed with PBSX1 and incubated with 30 μg/mL DSL-PMB ready made solution at room temperature. Images were acquired immediately after staining using Olympus BX53F2 upright microscope (X40 magnification). Image depicts PMB-DSL staining, excitation -360-370 nm, emission 420-460 nm (DAPI filter).
| availability | not available in China |
| concentration | 1.5 mg/mL in H2O |
| form | solution |
| Quality Level | 100  |
| shipped in | ambient |
| storage temp. | −20°C |
| suitability | suitable for (Fluorescent detection of gram-negative bacteria) |
| suitable for (Microbiome) | |
| suitable for microbiology |
| Analysis Note: | • Dansyl Labeled Polymyxin B Ready Made Solution is light sensitive. • It is recommended to avoid freeze-thaw cycles of PMB-DSL Ready Made Solution. • Dansyl Labeled Polymyxin B Ready Made Solution (1.5 mg/mL) can be diluted 1:50 in PBSX1 (Sigma#D8537) to achieve 30 μg/mL final concentration for staining. The above concentration of DSL-PMB was used for E. coli staining see image. • Fluorescence Microscopy application: Dansyl Labeled Polymyxin B Ready Made Solution excitation (Ex) wavelength is 330-340nm resulting in emission (Em) range of 540-600nm (λmax=570nm). |
| Application: | DSL-PMB may have been used:• as a fluorescent probe to study polymyxin mode of action and its pharmacokinetics • in research and to develop new active derivatives of Polymyxin against multi-drug resistance Gram-negative infections • to measure LPS binding affinity by fluorometric displacement assay Fluorescent antibiotics can be used for many applications including:• Antimicrobial resistance research. • Bacterial visualization and imaging. • Parent antibiotic mode of action research and new antibiotic discovery. • Toxicity studies. • Research of bacterial infections and tracking its uptake in vivo. |
| Biochem/physiol Actions: | DSL-PMB′s mode of action is in accordance with Polymyxin B activity. However, MIC assays comparing DSL-PMB, and parent Polymyxin B showed a two to three-fold decrease in bacterial activity. Polymyxin B interacts with anionic lipopolysaccharide (LPS) molecules which are located in Gram-negative bacterial outer membrane. This interaction interferes with the outer membrane′s normal function, causing leakage and enhanced uptake of the antibiotic. The resemblance in properties between Polymyxin B and DSL-PMB allows to fluorescently label Gram-negative bacteria and examine intracellular localization and penetration of Polymyxins in Gram-negative bacteria. |
| General description: | Dansyl Polymyxin B (DSL-PMB) is a fluorescent derivative of Polymyxin B. Polymyxin B is a cationic lipopeptide antibiotic that binds specifically to Gram-negative bacteria. Fluorescent antibiotics are obtained by synthetic conjugation of an antibiotic to a fluorophore. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |